Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Oxidized haematine solution and its preparing process and application

A hematoxylin semen and semen technology, applied in the field of oxidized hematoxylin semen and its preparation, can solve the problems of short storage period, complex processing technology, inconvenient use, etc., and achieve strong nuclear affinity specificity and good product stability , the effect of the simple method

Inactive Publication Date: 2004-12-22
韩亚京
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Hematoxylin semen is a staining reagent for histocytology. It is widely used in the fields of biomedical clinical pathology diagnosis, biomorphology teaching and research. In the past, the oxidized hematoxylin required for the preparation of hematoxylin semen was a solid product. The processing technology is complicated, it is temporarily prepared when necessary, it is inconvenient to use, the reagent staining selectivity is poor, the nuclear and interstitial components are not clearly differentiated, and its stability is not ideal, and the storage period is short. Therefore, it is necessary to provide a stable oxidized hematoxylin semen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oxidized haematine solution and its preparing process and application
  • Oxidized haematine solution and its preparing process and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Weigh 20 grams of hematoxylin solid powder, dissolve it in 100 milliliters of 80% ethanol, stir thoroughly to dissolve, add 4 grams of sodium iodate powder, stir fully at room temperature, and pour the reaction suspension into the reflux reactor During heating and boiling, the reflux time is 2 hours. At this time, the reaction solution is a dark brown-red transparent solution. Use a glass straw to absorb the reaction solution and apply it on a white quantitative filter paper. It is dark brown, and the reaction can also be checked by conventional thin layer chromatography. To a certain extent, the reaction can be terminated until a single dark brown-red spot (the formula of the chromatographic solution is 95% ethanol g 2ml, glacial acetic acid 18ml, and the chromatography silica gel is thin-layer chromatography silica gel G (type 60) can be terminated. Natural cooling to room temperature, Filter to obtain the filtrate, get 80 milliliters of glycerol and add in the filt...

Embodiment 2

[0028] 1. Weigh 10 grams of hematoxylin solid powder, dissolve it in 100 ml of 95% ethanol, stir thoroughly to dissolve it, add 2 grams of potassium iodate powder, stir fully at room temperature, and pour the above suspension into the reflux reactor , heated to boiling, refluxed for 3 hours, and other procedures were the same as in Example 1.

[0029] 2. Preparation of mordant solution

[0030] Take 2000 milliliters of distilled water, add 250 grams of potassium aluminum sulfate 12 water, fully dissolve, add 100 milliliters of glycerol, 1000 milliliters of 95% ethanol, 200 milliliters of glacial acetic acid, and mix well.

[0031] 3. Hematoxylin semen preparation

[0032] Take 3 parts of oxidized hematoxylin semen and add 7 parts of mordant solution, stir and mix well.

[0033] The hematoxylin semen (containing mordant solution) provided by the invention is used for cell smear staining, tissue section staining, and histochemical nuclear counterstaining. Reagents related to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An oxidized haematine solution, a dark brown transparent liquid, is prepared through adding iodate to the solution of haematine in alcohol, heating to boil, reflux reaction, filtering, and adding propanetriol to the filtrate. The resultant solution has stronger karyon affinity specificity, which can make the karyon show fine and clarly layered blue-black colour, but the tissue interstitial collagen fibre colour-less. Said solution has high stability.

Description

technical field [0001] The invention relates to a chemical reagent, in particular to an oxidized hematoxylin semen and its preparation method and application. Background technique [0002] Hematoxylin semen is a staining reagent for histocytology. It is widely used in the fields of biomedical clinical pathology diagnosis, biomorphology teaching and research. In the past, the oxidized hematoxylin required for the preparation of hematoxylin semen was a solid product. The processing technology is complicated, it is temporarily prepared when necessary, it is inconvenient to use, the reagent staining selectivity is poor, the nuclear and interstitial components are not clearly differentiated, and its stability is not ideal, and the storage period is short. Therefore, it is necessary to provide a stable Oxygenated hematoxylin semen. Contents of the invention [0003] The object of the present invention is to provide a kind of oxidized hematoxylin semen and its preparation method...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61B10/00C07D311/94G01N1/30G01N33/48
Inventor 韩亚京
Owner 韩亚京
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com