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Fowl castration recombinant gene vector

A technology for recombining genes and poultry, which is applied in the directions of genetic engineering, introduction of foreign genetic material using vectors, and plant gene improvement, etc., can solve the problems of low expression yield, high cost, complicated purification process, etc. simple effect

Inactive Publication Date: 2005-05-04
成都瑞盛高科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Prompting animals to produce anti-LHRH antibodies by immunization to neutralize the physiological effects of LHRH, thereby inhibiting the development of gonads to achieve the purpose of castration, its feasibility has been confirmed by many scholars at home and abroad, but most of the research has focused on the synthesis of Peptide vaccines, although the immune effect is remarkable, cannot be put into practical application due to the high cost
Wang Caiping expressed the LHRH / HBsAg fusion protein in the silkworm baculovirus, but it was not popularized and applied due to the low expression yield and cumbersome purification process

Method used

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  • Fowl castration recombinant gene vector
  • Fowl castration recombinant gene vector
  • Fowl castration recombinant gene vector

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Experimental program
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Effect test

Embodiment

[0065] 1. Design and artificial synthesis of LHRH gene

[0066] Design the gene according to the amino acid sequence of LHRH of birds and mammals, add NcoI and XhoI restriction sites and protective bases at the 5' end and 3' end respectively, and synthesize the gene with an automatic DNA synthesizer.

[0067] 1.1. Livestock vaccine LHRH gene sequence - L1:

[0068] 5′-GG CCATGG CTGAGCATTGGTCTTATGGCCTGCGCCCAGGT

[0069] TAATGA A CTCGAG CA-3′

[0070] terminator XhoI

[0071] 1.2. Poultry vaccine LHRH gene sequence - L2:

[0072] 5′-GG CCATGG CTGAGCACTGGTCTTACGGCCTGCAGCCGGGT

[0073] TAATGA A C TCGAG CA-3′

[0074] terminator XhoI

[0075] 1.3. LHRH Gene Sequence 1 of General Livestock / Poultry Vaccine - L3:

[0076] 5′-GG CCATGG CTGAACACTGGTCCTACGGTCTGCGTCCGGGT

[0077] GGCGGTGAGCACTGGTCTTACGGCCTGCAGCCGGGTGGCGGTGAACATTG

[0078] GTCTTACGGTCTGCGCCCGGGT TAATGA A CTCGAG CA-3′

[0079] terminator XhoI

[0080] 1.4. LHRH Gene Sequence...

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Abstract

The fowl farm animal castration vaccine gene and recombinant gene vector is used for producing farm animal castration gene engineering subunit vaccine and belongs to biological technology. The production process includes artificial synthesis of livestock, fowl or universal LHRH gene segment with NcoI and XhoI enzyme incision sites separately in its 5' end and 3' end; inserting pET-32c plasmid to constitute four kinds of recombinant pET-32c LHRHn / N / X plasmid to express LHRH-Trx fusion protein; and converting colibacillus BL21(DE3) with the recombinant plasmid to produce expression produce soluble protein. The farm animal castration gene engineering subunit vaccine is easy to purity, universal for livestock and fowl, and may be used for immunizing chicken, pig, sheep, rabbit, dog, can and other animal for effective castration.

Description

[0001] This application is a divisional application of application date: 2000.6.23, application number: 00107831.3, name: animal castration vaccine gene and genetic engineering body. (1) Technical field [0002] The animal castration recombinant gene carrier of the invention is used for producing luteinizing hormone releasing hormone (LHRH) genetically engineered subunit vaccines for castration of livestock and poultry, and belongs to the high-tech field of biotechnology. (2) Technical background [0003] Luteinizing hormore releasing hormone (LHRH) is a decapeptide hormone secreted by the hypothalamus of animals, and its main biological function is to control the synthesis and secretion of luteinizing hormone (LH) and Follicle hormone (FSH), these hormones can promote the normal development of gonads and sex organs, and maintain the reproductive function of animals. Castration vaccine can immune regulate the level of sex-related hormones in animal endocrine system, inhibit ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7088A61K39/00A61P15/00C07H21/04C12N15/13C12N15/16C12N15/63C12P19/34
Inventor 吉传义杜念兴朱杰青龚光明王凌龚晓明
Owner 成都瑞盛高科技股份有限公司