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Method for preparing alpha-fetoglobulin transspecific factor

A technology of alpha-fetoprotein and transfer factor, applied in the biological field, to achieve the effect of powerful immune regulation function

Inactive Publication Date: 2005-06-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, non-specific transfer factor and hepatitis B specific transfer factor have been applied clinically, with clear curative effect and no obvious adverse reactions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 A preparation method of alpha-fetoprotein-specific transfer factor of the present invention

[0025] 1. Preparation of immune antigen:

[0026] (1) Preparation of Freund's complete antigen: grind 15g of lanolin and 85ml of liquid paraffin oil, and then press high pressure for 30'. Take the above-mentioned incomplete adjuvant 1ml+BCG1mg Serve Freund's complete antigen.

[0027] (2) Antigen preparation for immunization: Take 1ml of alpha-fetoprotein (including 20ug) and add 1ml of complete Freund's adjuvant. First put the adjuvant in a sterile agate mortar, and add the antigen dropwise while grinding until the drop does not disperse on the water surface, forming a water-in-oil preparation.

[0028] 2. Immunization procedure:

[0029] Get healthy pigs (about 50 kilograms).

[0030] (1) The first immunization: 2ml of the antigen was injected subcutaneously at pig limbs, armpit, groin, country nest, etc. at multiple points, 0.2ml per point, with an interval of ...

Embodiment 2

[0039] Example 2 Partial application of alpha-fetoprotein-specific transfer factor

[0040] 1. The alpha-fetoprotein-specific transfer factor obtained by the method of the present invention can significantly reduce the tumor size of mice bearing liver cancer and prolong the survival time of the animal model.

[0041] 2. The alpha-fetoprotein-specific transfer factor obtained by using the patented technology of this invention can be made into injection preparations and oral preparations for the treatment of primary liver cancer, which has great social and economic benefits.

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PUM

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Abstract

The invention provides a method for preparing alpha-fetoprotein-specific transfer factor, which is the full-length or partial fragment of alpha-fetoprotein extracted from embryonic liver or expressed by genetic engineering The fragmented alpha-fetoprotein is used to immunize animals, the spleen and leukocytes of the immunized animals are collected, and the specific transfer factor is obtained by separation. The alpha-fetoprotein transfer factor prepared by the method has a powerful immune regulation function, and the prepared preparation can be used in the treatment of primary liver cancer and the auxiliary treatment of liver cancer. The preparation form of the alpha-fetoprotein specific transfer factor obtained by using the method of the present invention can be injection preparation and oral preparation.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a preparation method of transfer factor, in particular to a preparation method of alpha-fetoprotein-specific transfer factor. The prepared preparation can be used in the treatment of primary liver cancer and adjuvant therapy of tumors. Background technique [0002] Primary liver cancer is a common malignant tumor that endangers human health. Epidemiological survey found that primary liver cancer is closely related to chronic infection of HBV and HCV. my country is a high-incidence area of ​​viral hepatitis. At present, there are more than 100 million HBV and HCV infected people, which leads to a high incidence of liver cancer. According to statistics from the Ministry of Health, primary liver cancer has been the second leading cause of death among all tumors since the 1990s. Although the efforts of many oncology experts have achieved a variety of treatment methods such as sur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/395A61P35/00C07K16/18
Inventor 陈智朱海红周林福
Owner ZHEJIANG UNIV