Method for producing collagen oligopeptide
A collagen oligopeptide and collagen technology, applied in the field of enzyme catalysis, can solve problems such as increasing difficulty and product cost, and achieve the effect of reducing production cost and simplifying downstream processing technology
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Embodiment 1
[0032] Embodiment 1 (comparative example)
[0033] Use a knife to scrape the fat from fresh pork skin on the market, cut it into small pieces of 2×2 cm, and then grind it with a small civilian meat grinder. Get 12g of pretreated pigskin collagen (dry basis weight) and 200ml tap water and add in the fermentor with water jacket, firstly raise the temperature in the fermentor to 90 ℃ under stirring and keep for 5 minutes. Then cool down to a constant temperature of 60° C., and measure the pH value of the feed liquid in the fermenter to be about 6.8 by a pH meter. Then, 300 μl of Alcalase alkaline protease (2.4 L, labeled activity 2.4 AU / g, produced by Danish NOVO Company) was added to the fermenter, and hydrolysis was started under constant stirring. In this example, the substrate concentration was 60 g pig skin collagen / liter water and the enzyme to substrate ratio was 20 μl Alcalase alkaline enzyme (2.4 L) / g pig skin collagen. The total hydrolysis time was 12 hours. During t...
Embodiment 2
[0035] Repeat Example 1, but while adding 300 μl of Alcalase alkaline protease, add 4% (based on the substrate dry basis weight) of Aspergillus niger acid protease (enzyme activity 3000u / g). The analysis results are: the pH value of the hydrolyzed solution continues to decrease during the hydrolysis time, and reaches about 5.3 after 12 hours of hydrolysis. The substrate degradation rate reached the highest value of about 94.7% after 4 hours, and thereafter there was little change. The degree of protein hydrolysis continued to increase during the investigation time. When the hydrolysis time reached 12 hours, the degree of hydrolysis increased to about 17.7%. The oligopeptides with less than 10 amino acids accounted for about 40% of the peptide content in the hydrolyzate obtained by hydrolysis for 12 hours as measured by Sephadex chromatography.
Embodiment 3
[0037] Repeat Example 2, but the Aspergillus niger acid protease is added to the fermenter 1 hour after the Alcalase alkaline protease is added, and the temperature of the fermenter is adjusted to 55°C. Then the result of hydrolysis for 12 hours is: the pH value of the hydrolyzate is about 5.1, the degradation rate of the substrate is about 94%, and the degree of protein hydrolysis is about 16%.
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