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Arabidopsis thaliana BVD1 gene and its use

An Arabidopsis, gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of not being able to accurately control the absorption amount and location of exogenous hormones

Inactive Publication Date: 2005-08-31
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional physiological research methods cannot accurately control the amount and location of exogenous hormones absorbed by plants.

Method used

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  • Arabidopsis thaliana BVD1 gene and its use
  • Arabidopsis thaliana BVD1 gene and its use
  • Arabidopsis thaliana BVD1 gene and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0022] 1. Arabidopsis mutant bushy and dwarf 1 (bud1), the original wild-type material is Col-0 ecotype.

[0023] 2. Plant cultivation

[0024] Arabidopsis was cultivated with vermiculite poured with 1 / 3 B5 culture solution, the growth condition was continuous light at 23°C, and the intensity was 80-120μmol·m -2 sec -1 . The aseptic culture of Arabidopsis thaliana adopts surface sterilization. After 70% alcohol surface sterilization for 3-5 minutes, soak and sterilize with 10% Bleach for 10-15 minutes, and wash with sterile water for 3-4 times. Treat at 4°C for 4 days to promote growth after germination on 1 / 2 MS medium under the same conditions as vermiculite culture.

[0025] 3. Co-segregation experiment of bud1 and T-DNA

[0026] Genomic total DNA was extracted from 10 wild-type plants and 70 mutant plants from the F2 population of bud1 homozygous and wild-type Arabidopsis hybrids. Plant genomic DNA was extracted using the CTAB method created by Lijiayang in 1995. The...

Embodiment example 2

[0038] Agrobacterium tumefaciens-mediated transformation of Arabidopsis

[0039] The construction of the transformation plasmid overexpressing BUD1 was obtained by ligating the genomic PCR product of BUD1 between the BamH I and Sac I sites of pBI121, and the primers used were 5'-GGATCCTCTCTCTTCTATTTCCATGGC-3' and 5'-GAGCTCACAAGCAGTCGGATAAAG-3'. Transform the constructed transformation plasmid into the Agrobacterium GV3101 (pMP90) strain by electric shock method. After colony PCR identification, transform the Arabidopsis Col-0 wild type by the vacuum method established by Bechtold N1993. On the 1 / 2 MS medium of prime (50ng / l), when the T1 generation plants grow to 4-6 rosette leaves, move them into the soil, and if necessary, take a rosette leaf to extract genomic DNA for detection. After the single plant of the T1 generation was harvested, some seeds were screened with kanamycin to observe the segregation of the T2 generation, and some were sown in vermiculite to obtain stable...

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Abstract

The invention is genes cloned from bushy and dwarf 1 (bud1) which is testified that it has affected to plant IAA metabolism and function of controlling the plant form, it is named as BUD1. It can control the plant form by excessive expression. Amino acid series displays the gene is unknown MAPKK-AtMKK7 in MAPK cascade system. The high expression of BUD1 cause the apical dominance lost and dwarf form. The gene has a very important application value.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to a BUD1 (BUSHY AND DWARF 1) gene cloned by an activation labeling method, and the identification of the gene by a transgenic experiment; Source genes, related vectors, and the use of the genes or their functional analogs to regulate plant traits such as apical dominance and plant height. technical background [0002] The shape of a plant is largely determined by the branching system of the stem. The structure of the whole stem is jointly determined by the apical meristem of the main stem produced in the embryonic stage and other meristems produced in the late embryonic stage. The apical meristem of the main stem determines the main axis of the plant, and the side branches derived from other meristems further modify the configuration of the plant. The branching process of stems generally includes two developmental stages: the formation of ax...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00C07K14/415C12N15/29C12N15/63C12N15/70C12N15/82
Inventor 李家洋戴亚王晓群周亦华
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI