Self floculation hydrogen producing bacteria and its sieving method
A self-flocculation and bacterial technology, applied in the direction of bacteria, biochemical equipment and methods, applications, etc., can solve the problems of increasing operating costs, limiting hydrogen production efficiency, failing to meet hydrogen production and growth conditions, etc.
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[0017] The self-flocculating hydrogen-producing bacterium Harbin Ethanologen W-1 is named as Ethanologenbacterium Harbin W-1, and the preservation number is CGMCC 1151.
[0018] The method for screening self-flocculation hydrogen-producing bacteria Harbin ethanologen W-1 adopts conventional separation, purification and screening processes.
[0019] 1. Isolation, purification and screening of bacterial strains: the improved bacterial isolation medium was used to isolate hydrogen-producing bacteria. Take out 1-2mL of hydrogen-producing fermentation activated sludge from the continuous flow biological hydrogen production reactor CSTR, put it into a nitrogen-filled Erlenmeyer flask, add a few glass beads, shake it on a shaker for 1 hour, and beat the bacterial micelles in the sludge. crushed, diluted with sterile water, and then inoculated in solid medium to make rolling tubes, cultured for 7-10 days. Pick a single colony and transfer it to liquid medium. Observe whether there a...
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