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Self floculation hydrogen producing bacteria and its sieving method

A self-flocculation and bacterial technology, applied in the direction of bacteria, biochemical equipment and methods, applications, etc., can solve the problems of increasing operating costs, limiting hydrogen production efficiency, failing to meet hydrogen production and growth conditions, etc.

Active Publication Date: 2006-10-18
HARBIN INST OF TECH
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Problems solved by technology

[0004] Because the growth conditions of the hydrogen production reactor are different from those of the static culture, especially the pH of the reaction system during the acidogenic fermentation process is low, so although the high-efficiency fermentative bacteria isolated so far have high hydrogen production capacity, they cannot It often cannot achieve its optimal hydrogen production and growth conditions. High-efficiency hydrogen-producing bacteria cannot become the dominant population in the microbial community. They can only be immobilized, which limits their hydrogen production efficiency and increases operating costs.

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  • Self floculation hydrogen producing bacteria and its sieving method
  • Self floculation hydrogen producing bacteria and its sieving method

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Embodiment Construction

[0017] The self-flocculating hydrogen-producing bacterium Harbin Ethanologen W-1 is named as Ethanologenbacterium Harbin W-1, and the preservation number is CGMCC 1151.

[0018] The method for screening self-flocculation hydrogen-producing bacteria Harbin ethanologen W-1 adopts conventional separation, purification and screening processes.

[0019] 1. Isolation, purification and screening of bacterial strains: the improved bacterial isolation medium was used to isolate hydrogen-producing bacteria. Take out 1-2mL of hydrogen-producing fermentation activated sludge from the continuous flow biological hydrogen production reactor CSTR, put it into a nitrogen-filled Erlenmeyer flask, add a few glass beads, shake it on a shaker for 1 hour, and beat the bacterial micelles in the sludge. crushed, diluted with sterile water, and then inoculated in solid medium to make rolling tubes, cultured for 7-10 days. Pick a single colony and transfer it to liquid medium. Observe whether there a...

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Abstract

A self-flocculating hydrogen-producing bacterium relates to a new species of hydrogen-producing bacteria. In order to further improve the hydrogen production capacity of the system, it is necessary to separate the self-flocculating bacteria that can produce hydrogen efficiently, have strong acid resistance, and become a dominant growth, which has more practical significance and engineering application value. The self-flocculation hydrogen-producing bacterium of the present invention is named as Ethanologenbacterium Harbin W-1 (Ethanologenbacterium Harbin W-1), and the preservation number is CGMCC1151. The screening method of self-flocculation hydrogen-producing bacteria is characterized in that a new culture medium is used in the screening process. The self-flocculation hydrogen-producing bacterium Harbin Ethanologen W-1 of the present invention is a bacterium with high hydrogen production efficiency, strong flocculation ability and acid resistance. Body, 0.5-1.0mm in diameter, the medium is completely clarified.

Description

Technical field: [0001] The invention relates to a new species of hydrogen-producing bacteria. Background technique: [0002] Biological hydrogen production technology is one of the important means to solve the energy crisis and environmental pollution problems. Due to its dual efficacy and sustainability, it has been increasingly valued by countries all over the world. Improving the efficiency of hydrogen production is an urgent problem to be solved in the industrialization of biohydrogen production technology. Screening efficient hydrogen-producing bacteria is an effective way to accelerate the industrialization of biological hydrogen production. In order to obtain efficient hydrogen-producing fermentation bacteria, foreign researchers isolated a large number of hydrogen-producing fermentation bacteria, and most of the isolated hydrogen-producing fermentation strains were concentrated in Clostridium and Enterobacter. Kumar et al. isolated Enterobacter cloacae (Enterobact...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01C12N15/31
Inventor 邢德峰任南琪宫曼丽李秋波
Owner HARBIN INST OF TECH