Polycondensed amino-acid coordinated SOD simulatives

An amino acid and mimetic technology, applied in the field of superoxide dismutase analogs, can solve the problems of reduced capacity, poor lipid solubility, and reduced SOD-like activity, and achieves moderate molecular weight, broad application prospects, and good water solubility.

Inactive Publication Date: 2006-10-25
北京联合大学职业技术师范学院
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the SOD simulants prepared by these ligands also have the disadvantage of obviously poor water solubility, and some substances are even not good in fat solubility, which limits the practical application.
If organic solvents such as ethanol are used as auxiliary solv

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polycondensed amino-acid coordinated SOD simulatives
  • Polycondensed amino-acid coordinated SOD simulatives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Weigh 2.0 g of aspartic acid polycondensate with an average molecular weight of 3000 and dissolve it in 15 mL of 1 mol / L ammonia water. The solution is a dark red transparent solution, and the pH value is adjusted to 4 with 10 mol / L hydrochloric acid solution. In addition, 1.5 g of ferrous sulfate and equimolar hydroxylamine hydrochloride were weighed and dissolved in 15 mL of distilled water. Mix the two solutions in a 250mL ground-mouth Erlenmeyer flask. After mixing, the system is still a dark red transparent solution. Put the reaction system in a constant temperature water bath, gradually raise the temperature to 80°C, and take it out after magnetic stirring for 6 hours. The system is still deep red. Red transparent solution, cooled to room temperature. After overnight at 4°C, flocculent suspended matter appeared, and the precipitate after suction filtration was washed twice with distilled water, and dried naturally to obtain a light orange powder with a yield of ab...

Embodiment 2

[0026] Weigh 2.0 g of the aspartic acid polymer and dissolve it in 15 mL of 1 mol / L ammonia water, and adjust the pH value to 7 with 10 mol / L hydrochloric acid solution. Separately weigh 1.7 g of manganese sulfate and dissolve it in 15 mL of distilled water. The two solutions were mixed in a 250 mL ground-mouth Erlenmeyer flask. After mixing, the system was a deep red transparent solution. After magnetic stirring and reflux for 6 hours, the system was still a deep red transparent solution and cooled to room temperature. After overnight at 4°C, flocculent suspended matter appeared, and the filtered precipitate was washed twice with distilled water and air-dried naturally to obtain a white powder. The yield is about 60%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a kind of SOD simulant coordinated by polycondensed amino acid, and prepares a kind of polycondensed amino acid metal complex-polycondensed ferrous aspartate complex by using amino acid condensate as a ligand and different divalent metal ions in aqueous solution And polycondensed aspartic acid manganese complexes. Firstly, after dissolving polycondensed aspartic acid in ammonia water, add manganese sulfate solution under the condition of adjusting pH to be neutral to reflux reaction to obtain polycondensed aspartic acid manganese complex; add ferrous sulfate and ferrous sulfate under acidic conditions Equimolar hydroxylamine hydrochloride, reflux reaction to obtain polycondensation aspartic acid ferrous complex. The chemical composition and structure of the complex were characterized by elemental analysis and infrared spectroscopy, and its catalytic O 2 ・- SOD-like activity for disproportionation reactions. The SOD simulant of the invention has the characteristics of moderate molecular weight, good water solubility, non-toxicity, high activity, etc., and has broad application prospects.

Description

technical field [0001] The present invention relates to a superoxide dismutase (SOD) mimic. Background technique [0002] People have done a lot of research on natural superoxide dismutase (SOD), which can specifically remove superoxide anion free radicals (O 2 ·- ), so that it can be quickly transformed into non-toxic substances, thereby preventing active oxygen poisoning, anti-aging and reducing the occurrence of diseases such as cancer. However, due to the shortcomings of natural SOD, such as its large molecular weight, difficulty in permeating cell membranes, short half-life in vivo, alloantigenicity, and high price, its practical application is greatly restricted. [0003] Starting from the active center of SOD, using various ligands such as amino acids and Schiff bases to form complexes with metal ions such as Cu, Fe, and Mn to simulate SOD is a current research hotspot. In the work of simulating SOD, it has developed from the simple simulation of SOD active center ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/08A61K38/44
Inventor 李金刘京萍巫丽坤
Owner 北京联合大学职业技术师范学院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap