Preparation of temperature-sensitive chitosan-graft-NIPAAm/VL copolymer transgenic carrier
A temperature-sensitive chitosan and transgenic vector technology, applied in the field of gene therapy, can solve the problems of reducing the transfection rate, complex dissociation obstacles, etc. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0018] Dissolve 2 g of isopropylacrylamide in 25 ml of tetrahydrofuran, add 0.25 g of vinyl laurate, stir magnetically for 20 minutes, add 0.01 g of azobisisobutyronitrile and 20 μl of mercaptoacetic acid, and store at 45 °C under nitrogen protection. After reacting for 5 hours, the product was precipitated and washed three times with ether, and the sample was dried in a vacuum oven.
[0019]Get 2.0 gram of last step reaction gained samples and be dissolved in 20ml deionized water, get 3.5 grams of molecular weight 1000, the chitosan that deacetylation degree is 95% is dissolved in 20ml deionized water, add 0.15 gram carbodiimide, with hydrochloric acid / N, N, N', N'tetramethylethylenediamine adjusted the pH value to 4.5, reacted with magnetic stirring at 5°C for 6 hours, dialyzed the product in deionized water for 5 days, and freeze-dried to obtain the final carrier.
[0020] Dissolve 5 mg of the dialyzed thermosensitive carrier in 0.02M acetic acid / 0.02M sodium acetate solut...
Embodiment 2
[0022] Dissolve 2 g of isopropylacrylamide in 25 ml of tetrahydrofuran, add 0.98 g of vinyl laurate, stir magnetically for 20 minutes, add 0.02 g of azobisisobutyronitrile and 50 μl of mercaptoacetic acid, and store at 60 °C under nitrogen protection. After reacting for 6 hours, the product was precipitated and washed three times with ether, and the sample was dried in a vacuum oven.
[0023] Get 2.0 gram of last step reaction gained samples and be dissolved in 20ml deionized water, get 3.5 gram molecular weight 2000, the chitosan that deacetylation degree is 90% is dissolved in 20ml deionized water, add 0.35 gram carbodiimide, with hydrochloric acid / N, N, N', N'tetramethylethylenediamine adjusted the pH value to 5.0, reacted with magnetic stirring at 10°C for 8 hours, dialyzed the product in deionized water for 5 days, and freeze-dried to obtain the final carrier.
[0024] Dissolve the dialyzed 5mg temperature-sensitive carrier in 0.02M acetic acid / 0.02M sodium acetate solut...
Embodiment 3
[0026] Dissolve 2 g of isopropylacrylamide in 25 ml of tetrahydrofuran, add 1.96 g of vinyl laurate, stir magnetically for 20 minutes, add 0.03 g of azobisisobutyronitrile and 70 μl of mercaptoacetic acid, and store at 60 °C under nitrogen protection. After reacting for 7 hours, the product was precipitated and washed three times with ether, and the sample was dried in a vacuum oven.
[0027] Get 2.0 gram of last step reaction gained samples and be dissolved in 20ml deionized water, get 3.5 grams of molecular weight 5000, the chitosan that deacetylation degree is 80% is dissolved in 20ml deionized water, add 0.50 gram carbodiimide, with hydrochloric acid / N, N, N', N'tetramethylethylenediamine adjusted the pH value to 5.0, reacted with magnetic stirring at 15°C for 8 hours, dialyzed the product in deionized water for 5 days, and freeze-dried to obtain the final carrier.
[0028] The dialyzed 10mg thermosensitive chitosan was dissolved in 0.02M sodium acetate / 0.02M acetic acid ...
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle diameter | aaaaa | aaaaa |
| size | aaaaa | aaaaa |
| particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More