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Acid and choline-resistant separated strain of lactobacillus with ability of reducing and assimilating cholesterol

A technology of Lactobacillus acidophilus and Lactobacillus, applied to medical preparations containing active ingredients, buttermilk, bacteria, etc., can solve the problems of difficult maintenance of bacterial count, difficult survival, complex nutritional requirements, etc.

Inactive Publication Date: 2007-01-03
FOOD IND RES & DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, Lactobacillus acidophilus is a group of strains with complex nutritional requirements. Except that it is relatively stable in yoghurt, other commercially available lactic acid bacteria are produced in dry bacterial powder or other processed forms (powder, granule and tablet). shape) and made into products, the bacteria are not easy to survive at room temperature or refrigerated storage for a long time, and the number of bacteria is difficult to maintain at the initial storage number
Therefore, the actual number of bacteria contained in commercially available non-yoghurt milk products is often lower than the number of bacteria marked on the packaging

Method used

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  • Acid and choline-resistant separated strain of lactobacillus with ability of reducing and assimilating cholesterol
  • Acid and choline-resistant separated strain of lactobacillus with ability of reducing and assimilating cholesterol
  • Acid and choline-resistant separated strain of lactobacillus with ability of reducing and assimilating cholesterol

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Isolation and screening of embodiment 1 lactobacillus isolate

[0065] Materials and Methods:

[0066] 1. Medium and diluent:

[0067] 1. Screening medium:

[0068] (A) Tomato juice agar medium

[0069] Casein enzymatic hydrolyzate (Sigma, Louis, Mo, USA) 10g

[0070] Skimmed milk 10g

[0071] Tomato Juice 400ml

[0072] Agar 12g

[0073] Distilled water up to 1L

[0074] (B) Rogosa agar medium

[0075] Rogosa agar (Merck, Darmstadt, Germany) 74.5 g

[0076] Distilled water 1L

[0077] After dissolving by microwave heating, cool to 55°C, adjust the pH value to pH 5.5 with acetic acid (C) Lactobacillus selective medium (LBS)

[0078] Rogosa agar 8.4g

[0079] Tomato Juice 40ml

[0080] Distilled water 60ml

[0081] After dissolving by microwave heating, cool to 55°C, adjust the pH value to pH 5.5 with acetic acid (D) Rogosa+X-glu agar medium

[0082] 80 mg of 5-bromo-4-chloro-3-indolyl 1-β-D-glucopyranoside (X-glu) (Sigma, Louis, MO, USA) was added to 100 m...

Embodiment 2

[0127] Embodiment 2 acid resistance test

[0128] 1. Experimental operation procedure:

[0129] With reference to the method disclosed in J.E.Holcombe et al., (1991), Cult.Dairg Prod.J., 26(3):4-5 and US 5,711,977 granted to Y.S.Yang et al., neutral (pH 7) and imitative gastric acid Environment (pH 2) to test the number of surviving bacteria.

[0130] After each test strain was activated twice with MRS broth, get its bacterium solution and centrifuge (3000rpm, 10 minutes), after removing the supernatant, add 1m1 of normal saline solution (0.85%NaCl, pH 7), first with a bamboo stick Stir the bacteria, shake them evenly, and then take 0.5ml of the dispersed and floated bacteria solution and put them in the physiological saline solution of pH 7 and pH 2 respectively. number of surviving bacteria. The smaller the value of Δlog bacterial count [= log (bacterial count at pH 7 - bacterial count at pH 2)], the strain with better acid resistance is considered.

[0131] 2. Results: ...

Embodiment 3

[0135] Example 3. Bile Salt Tolerance Test

[0136] 1. Experimental operation procedure:

[0137] After each test strain was activated twice with MRS broth, 1% of the inoculum was inoculated into 10ml MRS broth and 10mL MRS broth (MRSO) containing 0.3% ox bile, and cultured at 37°C for 24 hours. Spectrophotometer was used to measure the bacterial concentration (OD 660 ). In addition, the number of remaining bacteria was also counted, where Δlog bacteria value=log(number of bacteria in MRS broth-number of bacteria in MRSO broth), and the smaller the number of Δlog bacteria, the better the bile salt tolerance of the test strain.

[0138] 2. Results:

[0139] After the lactic acid bacteria are treated with bile salts, the survival rate of different strains is very different. Therefore, it is important to screen the lactic acid bacteria strains with tolerance to bile salts in the selection of probiotics (S.E.Gilliland et al., (1984), J . Dairy Sci., 67: 3045-3051; P. Marteau e...

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Abstract

The invention opened a new Lactobacillus which can resist the cholate, the gastric acid and assimilate the cholesterol. The strain or the progeny can be used to many kinds of the foods; and it can be used as the medicine for curing the gastrointestinal sickness and decreasing the cholesterol of the serum.

Description

[0001] This application is a divisional application of the Chinese patent application 02146947.4 submitted on October 30, 2002, and the title of the invention is "Novel acid-resistant and bile-resistant Lactobacillus isolates with the ability to reduce and assimilate cholesterol". technical field [0002] The present invention relates to a novel Lactobacillus isolated strain with bile-salt resistance and gastric-acid resistance and simultaneously capable of lowering serum cholesterol and its application. Background technique [0003] "Lactic acid bacteria" are a group of bacteria that can ferment sugars and produce lactic acid as the main product. The currently widely accepted morphological and physiological characteristics are: (1) Gram-positive bacteria; (2) Cocci in appearance or bacillus; (3) catalase test is negative; (4) can produce more than 50% lactic acid from the glucose it metabolizes; (5) does not form endophytic spores; and (6) is mostly non-swimming, Can grow u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/225A23L3/3463A23C17/02A23C9/127A23C13/16A23C19/032A23G9/36A61K35/74A61P3/06A61K35/747
Inventor 刘玉茹游金珠谭静芬廖啓成
Owner FOOD IND RES & DEV INST
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