Chuanxiongzine modified anticoagulant fimbrin material and its preparing method

A technology of silk fibroin and ligustrazine, applied in the field of biomedical materials

Inactive Publication Date: 2007-02-07
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the mid-1970s, Chinese herbal medicines such as Ligustrazine and its active ingredients have been used as anticoagulants to chemically modify materials, and there have been no reports.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0012] (1) Preparation of side chain functionalized ligustrazine

[0013] Prepare 2-formic acid-3,5,6-trimethylpyridine according to the literature (see "Synthesis of Metabolites of Ligustrazine in vivo [J]" Journal of Shandong Medical University, 1997, 1, 65; Author: Liu Xinyong et al. [1]) 6.3 grams of potassium permanganate were dissolved in 100 milliliters of water, and 2.7 grams of tetramethylpyrazine were dissolved in 20 milliliters of water. The two aqueous solutions were mixed and reacted at 70° C. for 2 hours. Filter and adjust the filtrate to acidic with hydrochloric acid. After standing still, crystals were precipitated. The measured melting point is 116-120°C. FTIR (KBr): 1720cm -1 (C=O, carbonyl in formic acid), 1436cm -1 (-N=C-, pyrazine ring).

[0014] (2) Preparation of silk fibroin film

[0015] Silk in 1% NaHCO 3 Boil in the solution to remove gum, dissolve in CaCl at 80°C 2 : C2H5OH: H2O=1: 2: 8 (molar ratio) solution and then dialyzed for three day...

preparation example 2

[0024] (1) The preparation method of side chain functionalized ligustrazine is the same as that of Preparation Example 1.

[0025] (2) Preparation of silk fibroin / Ligustrazine blend film

[0026] The preparation method of silk fibroin solution is the same as Preparation Example 1. Mix 20 milliliters of silk fibroin solution with 1 milliliter of 4% (w / v) ligustrazine aqueous solution, stir, pour into a petri dish, and form a film overnight in a constant temperature drying oven at 50°C.

[0027] (3) Surface chemical modification of silk fibroin / ligustrazine blend film

[0028] 1 g of 2-formic acid-3,5,6-trimethylpyrazine and 5 g of DCCI were dissolved in 100 ml of DMF, and an appropriate amount of silk fibroin / ligustrazine blend film was added, and electromagnetically stirred. After reacting at room temperature for 4 days, the silk fibroin film was taken out and washed with water several times, and dried at room temperature. ATR-FTIR: 1720cm -1 ~1750cm -1 (C=O) (ester bond)...

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PUM

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Abstract

The present invention provides a new kind of Chinese medicine modified anticoagulant polymer material, and is chuanxiongzine modified anticoagulant fimbrin material. The present invention also provides the preparation process of the chuanxiongzine modified anticoagulant fimbrin material. The Chuanxiong derivative with side chain converted into active radical is ester bond combined with fimbrin under the action of DCCI. The chuanxiongzine modified anticoagulant fimbrin material has improved anticoagulant activity, lowered water solubility and raised mechanical strength.

Description

technical field [0001] The invention relates to the research on ligustrazine-modified silk fibroin anticoagulant material and its preparation method, which is an effective component of Ligusticum chuanxiong, and relates to the field of biomedical materials. Background technique [0002] Silk fibroin is a natural polymer material and the main component of silk. Due to its good biocompatibility, it is widely used in the fields of biomedical materials such as enzyme immobilization technology, cell culture substrate, artificial skin, and drug sustained release materials. However, unmodified silk fibroin cannot meet the requirements of the environment in direct contact with blood. Chinese herbal medicine is a treasure of Chinese culture, and its application has a history of thousands of years. Clinical treatment and pharmacological experimental studies have shown that Ligustrazine, an active ingredient of the Chinese herbal medicine Ligusticum Chuanxiong for promoting blood cir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L33/12A61L33/00
Inventor 王松朱鹤孙
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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