Method for preparing extracts of rabdosia effective ingredient
The invention relates to a technology for rubescens and extracts, which is applied in the field of quantitative preparation of active ingredient extracts of rubescens, can solve the problems of poor quality controllability of Chinese patent medicines, lack of continuous process integrated extraction process for active ingredients, and the like, and solves the problem of solvent Residue and pollution problems, high level of equipment integration, high production recovery effect
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Embodiment 1
[0061]Select 200g of Rassonica raw material, crush it, add 5 times the amount of 85% ethanol to extract 3 times, each time of extraction is 2 hours, and obtain the extract of Russin sativa. The ethanol was recovered to obtain 12.3 g of green-brown Radix japonicus extract, which was dissolved by adding 50 ml of absolute ethanol, and then extracted by adding ethyl acetate to obtain the extract.
[0062] Get ethyl acetate extract, add 4 times of sherwood oil to carry out precipitation, obtain precipitate, add 50ml ethanol to it and dissolve, get the silica gel column on the dissolving solution, adopt sherwood oil: ethyl acetate=(10:1)-( 1:1) Mixed solvents were used for gradient elution at 12ml / min, and TLC and HPLC online tracking were performed at the same time, eluents were collected separately from different parts, and the required components were combined to obtain eluents. The eluate collected from each part was recovered to 1 / 3 volume, and 4 g of powdered activated carbon ...
Embodiment 2
[0065] Select 1000g of Rasmus japonicus raw material, pulverize it, add 6 times the amount of 90% ethanol to extract 3 times, each extraction time is 3 hours, and obtain the extract of Russin japonicus japonicus. The ethanol was recovered to obtain 62.8 g of green-brown Radix Grass extract, which was dissolved by adding 300 ml of absolute ethanol, and then extracted by adding ethyl acetate to obtain the extract.
[0066] Get ethyl acetate extract, add 4 times of sherwood oil to carry out precipitation, obtain precipitate, add 300ml ethanol to it and dissolve, get the silica gel column on the dissolving solution, adopt sherwood oil: ethyl acetate=(8:1)-( 1:4) gradient elution with a mixed solvent of 10ml / min, and online tracking by TLC and HPLC at the same time, separate eluents were collected at different parts, and the required components were combined to obtain eluents. The eluate collected from each part was recovered to 1 / 3 volume, and 10 g of powdered activated carbon was...
Embodiment 3
[0069] Select 3000g of Rasmus japonicus raw material, pulverize it, add 5 times the amount of 95% ethanol to extract 4 times, each extraction time is 3 hours, and obtain the extract of Russin japonicus japonicus. The ethanol was recovered to obtain 192.6 g of green-brown Radix japonicus extract, which was dissolved by adding 1000 ml of absolute ethanol, and then extracted by adding ethyl acetate to obtain the extract.
[0070] Get ethyl acetate extract, add 5 times of sherwood oil to carry out precipitation, obtain precipitate, add 800ml ethyl acetate to it and dissolve, get the silica gel column on the dissolving solution, adopt sherwood oil: ethyl acetate=(4: 1) -(1:3) mixed solvent was used for gradient elution at 15ml / min, and TLC and HPLC online tracking were performed at the same time, and eluents were collected separately at different parts to obtain eluents. The eluate collected from each part was recovered to 1 / 4 volume, and 50 g of powdered activated carbon was added...
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