Enzyme linked spot automatic detection instrument and its image analysis

Abstract

The invention belongs to electronic medical instrument. Carrier platform is movable along X, Y directions based on orthogonal two rolling guides. A flat plate having 96 holes for viewing spots of joining enzyme is fixed on upper plane of platform on cabinet for controlling platform. Motion control card and image acquisition card are inserted to computer. The motion control card through data wires is connected to connection board inside the cabinet, and sends signal to motor driver to bring carrier platform to desire position. Image acquisition card through data wires is connected to color camera to pick up static images to be transferred to computer. Advantages are high work efficiency and good measuring precision and stable system.

Description

Technical field [0001] The invention belongs to the field of electronic medical instruments, and particularly relates to an enzyme-linked spot detection automatic instrument for biochemical analysis and image analysis thereof. Background technique [0002] As a biochemical analysis device, it includes various automatic or semi-automatic biochemical analyzers. Their common feature is to extract and detect certain characteristics of human biochemistry, and use a computer (microprocessor) for analysis and processing. The enzyme-linked spot automatic detector is an enzyme-linked enzyme-linked instrument that performs image capture and data analysis for organ transplantation, vaccine and drug research and testing, autoimmune research and testing, tumor and infectious disease research, etc. Equipment for spot experiment and detection. [0003] This instrument is a device that integrates motion control, image acquisition and image analysis, and is the first in China. In the known techno...

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Problems solved by technology

[0004] The technical problem to be solved in the present invention is that in the known technology, when performing enzyme-linked spot detection and analysis, it can only rely on separate specialized equipment, and a set of motion control, image acquisition and image analysis is provided. The integrated automatic control integrated instrument makes the detection and analysis work of this project cumbersome, inefficient and poor in accuracy. In order to overcome these defects, it is necessary to design an integral automatic detection analyzer for enzyme-linked spots. In order to achieve this The purpose of the invention is achieved by adopting the following technical solutions. An automatic enzyme-linked spot detector mainly includes an instrument device base, an object loading platform, a monocular stereo microscope and its lifting frame, a cold light source, a color camera and connected to it. The computer system is characterized in that the loading platform can move in x and y directions on the plane of the base of the device by relying on two orthogonal rolling guide rails installed at the bottom, and a control platform chassis is provided on one side of the loading platform. A 96-well flat plate for observing enzyme-linked spots is fixed on the upper plane, and the motion control card and image acquisition card for controlling the movement of the platform are inserted on the computer motherboard at the same time. The board sends a signal to the motor driver to drive the loading platform to move freely to the set position; the image acquisition card is connected to the color camera through the data line, and the current static picture collected is transmitted to the computer, and the shooting software saves the collected image to the hard disk directory middle

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