Cooperative fermentation process of bacterial enzyme prepn used as feed additive

Abstract

The invention relates to a associated fermentation technique of germzymin acted as fodder additive. Its process flow is as follows: to plant multiple bacterial spawn of enzyme production and multipleready produced bacterial spawn on the slope respectively, after ripeness to be developed into spore suspension; then mixing said of each kind respectively according to proportion; hence seed culture medium to be led into enzyme production and spore suspension of ready produced and mixed bacteria spawn; finally through developing seed culture medium sets up full spore, mixing seed disinfectant of enzyme production germ and of ready produced bacterium, developing into mixture transfer agent of germ and enzyme, thereafter ferment breeding, products after ferment to be treated and become germzymin.

Description

technical field [0001] The invention relates to a fermentation process, mainly a collaborative fermentation process of bacterial enzyme preparations used as feed additives. Background technique [0002] After more than ten years of research and development, the research, development and application of feed enzyme preparations in my country have made great progress. Especially in recent years, with the growing shortage of feed resources, it will become an inevitable trend to use wheat and rice grains as feed resources, and special enzymes such as xylanase, xylanase, and β-glucanase has become a research hotspot of feed enzyme preparations. Microecological preparations such as probiotics are used as biological feed additives in the feed industry, and great progress has been made in my country. Different types of probiotic preparations have appeared on the market in Sichuan, Shandong and other provinces in China, and have shown good results in practica...

AI Technical Summary

Problems solved by technology

At present, all kinds of strains used for the production of feed enzymes basically adopt traditional breeding methods for mutagenesis and selection. The enzyme production level of the strains cannot be greatly improved, and the stress resistance performance of the enzymes such as high temperature resistance cannot be improved. qualitative change

2) Single fermentation method

The current production of feed enzymes mostly uses a single strain of bacteria. Under solid culture conditions, one strain produces multiple enzymes to make a so-called "composite enzyme", resulting in incomplete or unreasonable enzymes in the product, and the production cost of the enzyme higher

3) The strains of probiotics are not comprehensive, and their products are easily inactivated during transportation, use and storage, thereby reducing biological activity

4) Feed enzymes and probiotics are fermented and produced separately, and then compounded together when used, which increases the production cost of these two microbial preparations, resulting in the inability to expand the application range

However, due to factors such as price and benefit, it is rare to add two kinds of microbial preparations to the feed at the same time, thus limiting the synergistic effect of the two preparations