Antibacterial peptide gene of Chinese prawn and its colon technique

A technology of Chinese prawns and antimicrobial peptides, applied in the field of molecular biology, can solve the problems that antimicrobial peptides have not been reported yet

Inactive Publication Date: 2002-11-27
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many researches on insect antimicrobial peptides in China. The expression of limulus antifungal peptides in Limulus has also been reported, and gratifying results have been achieved in antimicrobial peptide transgenic plants, but the research on antimicrobial peptides from crustaceans has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1. A cloned Chinese prawn antimicrobial peptide gene has the following sequence:

[0040] Sequence Listing Homologous Gene 1: (1) Information of SEQ ID NO 1 (a) Sequence Features:

[0041] *Length: 600 bp

[0042] *Type: nucleic acid

[0043] * Chain type: double chain

[0044] *拓扑结构:线性(b)分子类型:cDNA (c)假设:否(d)反义:否(e)最初来源:对虾(Penaeus chinensis) (f)序列描述:SEQ ID NO.1ATGCGCCTCGTGGTCTGCCTGGTCTTCTTGGCCTCCTTCGCCCTGGTCTGCCAAGGGCAA 60AAGGGTGGTTACACACGCCCGATATCCAGACCACCCTATGGGGGAGGATATGGCAATGTT 120TGCACTTCATGCCACGTTCTTACCACCTCACAAGCTCGTTCTTGCTGCAGTCGGTTTGGA 180CGTTGCTGTGTGCCAAGAAGAGGATACAGTGGTTGATGAAGAAGACAACGAAAACCTGAC 240TTCACAACGTATTCATTAATTGTGAAGAGACTGCAACCCTGATTTTGAACTGTATTTTCT 300CGTTCCATTTTTTTTACTTTTGCTTGTGGAAAGGATGCTTTGCAAGGATGCACTAAAGAT 360TTTTCCATGATTGCCTGATGAATGAAAGTGCATGTGGGATGTAAGTGCATTTGTCCCAGC 420AGGTCCTCGTGTATTCATGATTTGTAACACACGAGAAAGAATACTTGTTGTTTGACTTTC 480ATTGTAATTAATTGTACGCATGGGTCTGTGTGTGGTTGGT...

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PUM

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Abstract

An antibacterial peptide gene of Chinese prawn and its cloning process are disclosed. Its gene sequence features 600 base pairs for length, nucleic acid type, dual chains, and linear topology structure. The said antibacterial peptide gene is cloned from Chinese prawn by RT-PCR or Nested-PCR reaction, 3' RACE method, and smart cDNA method, and can be used for recombinant expression of the antibacterial peptide gene and its transfer, and so for preventing and treating diseases of prawn.

Description

(1) Technical field [0001] The invention relates to a Chinese prawn antimicrobial peptide gene and cloning technology, belonging to the technical field of molecular biology. (2) Background technology [0002] The immune response of insects / arthropods is a very sensitive system and thus can effectively resist the invasion of external microorganisms. Their immune response mainly depends on three reactions: proteolytic cascade reaction, that is, the rapid immune response caused by animal injury stimulation, including blood agglutination reaction, phenoloxidase cascade reaction and opsonization; blood cell phagocytosis of invading microorganisms and enveloping effect; after being stimulated, a large number of antimicrobial peptides or polypeptides are rapidly and instantaneously synthesized, and these molecules are mainly synthesized by fat body or blood cells, and are rapidly released into the hemolymph. Since the discovery of the first antimicrobial peptide in an insect, to d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12P19/34
Inventor 王金星赵小凡相建海
Owner SHANDONG UNIV
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