Liquid-solid combined fermentation process of probiotics by using bifidobacteria as main component

A technology of bifidobacteria bifidum and microecological preparations, applied in the field of microorganisms, can solve the problems of reduction of living cells, small number of cells, large power consumption, etc., and achieve the effect of prolonging the shelf life and promoting proliferation.

Inactive Publication Date: 2003-03-05
李盛学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this simple liquid (single liquid) culture has a small number of cells in the final preparation, up to 1 billion CFU/g. After liquid culture, starch and glucose are used as adsorption carriers, which is not conducive to the preservation of live bacteria. The liquid culture is directly freeze-dried , when the culture loses water

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Bifidobacterium bifidum

[0063] (1) Original seed liquid culture

[0064] Take 3.0g soybean peptone, 3.0g tryptone, 8.0g yeast extract, 8.0g glucose, 30ml trace salt solution, in which CaCl 2 0.1g, MgSO 4 ·7H 2 O 0.3g, K 2 HPO 4 0.5g, NaHCO 3 5.0g, NaCl 1.0g, distilled water 500ml, pH 6.5 as the culture medium. Put the medium into a test tube (accounting for about 2 / 3 of the vertical length of the test tube) and stuff it with a cotton ball, sterilize at 0.14MPa for 30min, and immediately replace it with a sterilized rubber stopper. Immediately inoculate the original seed with ice water to 45 ° C, and pour sterilized edible vegetable oil on the medium to make it a 0.5-1.0 cm thick oil layer, seal with the original rubber stopper, and put it in anaerobic environment and 37 After culturing at ℃ for 24-48h, the culture was terminated. Store them under anaerobic conditions at 0-4°C for later use.

[0065] (2) Liquid culture of primary strains

[0066] Take 6....

Embodiment 2-3

[0088] Bifidobacterium bifidum, Lactobacillus acidophilus and Streptococcus thermophilus at each stage of cultivation operations are the same as in Example 1, and the composition of their respective culture media. The proportions of the above three strains in the final microecological preparation are shown in Tables 9-15 respectively.

[0089]

[0090]

[0091] skim

[0092] cool eggs

[0093] cool eggs

[0094] Defatted Soybean Flour

[0095] Bifidobacterium bifidum

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PUM

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Abstract

The present invention belongs to the field of microbial technology, relates to a liquid-solid combined fermentation process of probiotics using bifidobacterium as main raw material, mainly includes the following steps: making bifidobacterium, acidophilic lactobacillus and thermophilic streptococcus respectively undergo the two stages of stock 1-3 order liquid culture and solid culture, the weightratio of three bacteria powders in the preparation is 50-80:10-15:5-16. Said solid culture includes the following procedures: firstly, sterilizing, inoculating 20-30% of tertiary culture, placing them into plastic bag, vacuum-pumping, injection anaerobic sterile N2 into said non-toxic sterile plastic bag, and adding bifiobacterium into solid culture medium, and promoting live bacterium multiplication to 10 to the power 8-10 to the power 9 order, and can prolong its storage time.

Description

technical field [0001] The invention belongs to the field of microorganisms and relates to a liquid-solid combined fermentation process using Bifidobacterium bifidum as the main microecological preparation. In particular, it relates to the liquid-solid combined fermentation process of the microecological preparations of Bifidobacterium bifidum, Lactobacillus acidophilus and Streptococus Salivarius subspthermophilus Background technique [0002] At present, there are literature reports on the liquid culture of Bifidobacterium bifidum and Lactobacillus acidophilus. However, this simple liquid (single liquid) culture has a small number of cells in the final preparation, up to 1 billion CFU / g. After liquid culture, starch and glucose are used as adsorption carriers, which is not conducive to the preservation of live bacteria. The liquid culture is directly freeze-dried , When the culture loses water, the synthetic osmotic pressure of the residue ...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 王厚德李盛学
Owner 李盛学
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