Carrier used for synthesizing nucleic acid and its creation method and application

Abstract

A cynthetic carrier instead of the controllable-pore glass beads for synthesizing nucleic acid is a nano-class paramagnetic microparticle of iron oxide, which is prepared by microemulsifying. A process for preparing nucleic acid from said microparticles includes polymerizing a layer of high-molecular organic compound on the surface of said microparticles, modifying it one or more times to generate aminomethyl, and synthesizing nucleic acid by triester phosphite method or synthesizing peptide nucleic acid (PNA) or polypeptide on tis surface. Its advantages are high specific surface and high output.

Description

technical field [0001] The invention relates to a nucleic acid synthesis carrier. The principle is to use the huge specific surface area of ​​nanoparticles as a solid phase support for nucleic acid synthesis. The nucleic acid synthesis carrier is used for nucleic acid to improve the output and yield of nucleic acid synthesis. Background technique [0002] With the deepening of genome research, it will become possible to understand the differences in life, the rules of disease occurrence and development, and the interaction between drugs and living organisms at the genetic level. The high-throughput detection and analysis technology of nucleic acid sequence information will become one of the core technologies in the field of life sciences such as medicine. People need to develop high-throughput, accurate, and low-cost genetic information detection methods. Recently, nucleic acid synthesis technology has attracted more and more attention. However, at present, nucleic acid sy...

AI Technical Summary

Problems solved by technology

However, because the use of large-pore carriers is limited in the scale of synthesis, it also limits the cost of increasing the scale of synthesis

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