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Testing mass spectrum model of blood serum protein in carcinoma of large intestine and its creating method

A mass spectrometry model and construction method technology, applied in mass spectrometers, measuring devices, biological tests, etc., can solve the problems of high false positive rate and false negative rate, achieve high specificity and sensitivity, improve cure rate, and reasonably design Effect

Inactive Publication Date: 2003-10-01
SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that there are many methods for the early diagnosis of colorectal cancer, but there is a contradiction between specificity and sensitivity, and the problem of high false positive rate and false negative rate

Method used

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  • Testing mass spectrum model of blood serum protein in carcinoma of large intestine and its creating method
  • Testing mass spectrum model of blood serum protein in carcinoma of large intestine and its creating method
  • Testing mass spectrum model of blood serum protein in carcinoma of large intestine and its creating method

Examples

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Effect test

Embodiment 1

[0019] Example 1 A mass spectrometry model and construction method for detecting colorectal cancer serum proteins of the present invention:

[0020] 1. Technical route:

[0021] Serum samples were thawed in an ice bath and centrifuged at 3000rpm for 5 minutes, 10ul was taken and added to 90ul 0.5% CHAPS (pH7.4) solution and mixed well (10 minutes). Albumin affinity resin (Cibacron Blue3GA (Sigma)) was pre-treated Equilibrate with 0.5% CHAPS 3 times x 5 minutes, place Cibacron Blue 3GA and serum samples on a shaker at 4°C for 60 minutes, centrifuge at 12000rpm for 5 minutes (4°C), and take 40ul of the supernatant solution. H4 chip (Ciphergen) was pre-equilibrated with 20mM HEPES (pH7.4) for 2 times × 5 minutes, the serum sample after removing albumin was diluted to 200ul with 20mM HEPES (pH7.4), and added to a 96-well Bioprocessor (Ciphergen) , shake on a shaking platform for 60 minutes. Shake off the serum sample, wash the chip with 200ul of 20mM HEPES (pH7.4) 3 times for 5 ...

Embodiment 2

[0027] Embodiment 2 Partial examples

[0028] 1. Samples and instruments:

[0029]Serum samples from 147 cases, including 55 cases of colorectal cancer, were obtained from the Oncology Department of the Second Affiliated Hospital of Zhejiang University School of Medicine, and 92 cases of healthy people were collected from healthy people who underwent physical examination in our hospital. All 55 cases of colorectal cancer were identified by postoperative pathological reports, including: Dukes A8 cases; Dukes B20 cases; Dukes C15 cases; Dukes D12 cases. Among them, there were 35 males and 20 females, with an average age of 57.6 years (31-84 years). 92 cases of healthy people were confirmed by routine physical examination items, matched by age and gender, including 60 cases of males and 32 cases of females, with an average age of 55.3 years (28-78 years). All serum samples were taken in the morning on an empty stomach, and the serum was separated and stored in a -80°C low-tempe...

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Abstract

A mass-sectrum model for detecting the serum protein of intestinal cancer, that is, non-intrusive detection of human intestinal cancer is composed of human serum protein mass-spectrum, mass spectrometer, and artificial nerve network analysis. Its configuration method includes preparing serum, collection of mass-spectrum data, and artificial nerve network analysis of mass-spectrum data. Its advantages are high sensitivity (more than 90%) and high specificity (more than 90%).

Description

Technical field [0001] The invention relates to the field of malignant tumor detection, and is a new non-invasive detection method for early detection of colorectal cancer in vitro, and the sensitivity and specificity of the early detection can reach more than 90%. Background technique [0002] The morbidity and mortality of colorectal cancer are on the rise in my country. According to the statistics recently published by the National Cancer Prevention and Control Office, colorectal cancer is ranked as the fourth major malignant tumor in terms of adjusted mortality rate, and its incidence rate in big cities is the third among males. place, women are the second place. According to the changes in the spectrum of digestive tract cancer in Europe, America, Japan and other countries in the past few decades, it can be predicted that colorectal cancer may become one of the most common malignant tumors in my country in the 21st century. Currently, 2 / 3 of the patients are middle and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/22G01N33/574G01N33/68G01N33/96H01J49/26
Inventor 陈益定郑树胡汛俞捷凯
Owner SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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