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Technique method for preparing hydratase of carbonitrile mitrile by using glucose-CO2+ coupling adding ferment

A technology of nitrile hydratase and process method, applied in the preparation, fermentation, hydrolase and other directions of organic compounds, can solve the problems of inconvenient on-line measurement, abnormal metabolism, difficult control and the like

Inactive Publication Date: 2003-10-08
TSINGHUA UNIV
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Problems solved by technology

This culture method is simple to operate, but it also has obvious disadvantages: in order to meet the demand for nutrients throughout the fermentation cycle, a relatively excessive amount of medium is added in the early stage of fermentation, and the higher glucose concentration will inhibit the growth of the bacteria, and The consumption of glucose in the fermentation is easy to produce acetic acid, which will acidify the fermentation broth. Too low pH will cause metabolic disorders of various enzymes in the cell, affect the utilization rate of the substrate by the bacteria, and is not conducive to the growth of the bacteria and the nitrile hydratase Synthesis
But Co 2+ The concentration of the concentration is not convenient for online determination, resulting in the addition of Co 2+ The amount is difficult to control, too much or too little is not conducive to the improvement of nitrile hydratase activity

Method used

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  • Technique method for preparing hydratase of carbonitrile mitrile by using glucose-CO2+ coupling adding ferment
  • Technique method for preparing hydratase of carbonitrile mitrile by using glucose-CO2+ coupling adding ferment
  • Technique method for preparing hydratase of carbonitrile mitrile by using glucose-CO2+ coupling adding ferment

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Embodiment Construction

[0014] We investigated glucose consumption and Co 2+ The impact on the enzyme production process, it is found that the maximum sugar consumption rate of glucose in the fermentation process appears in the rapid enzyme production period, and the enzyme production rate reaches the maximum. It is used to produce enzymes and maintain the metabolism of bacteria. From the perspective of changing trends, the enzyme production rate and sugar consumption rate show a high positive correlation at this stage. During the deceleration period of enzyme production, glucose tends to be exhausted, the rate of sugar consumption decreases, and the rate of enzyme production also decreases rapidly, and the trend of change is consistent. If the glucose concentration is regulated in the late stage of rapid enzyme production, so that the enzyme production rate can be maintained at a high level for a longer period of time, the enzyme production process can be further enhanced. On this basis, we develop...

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Abstract

A process for preparing nitrile hydratase from glucose and Co ions includes coupling glucose with Co ions and fermenting, and features that the output of nitrile hydratase is positivity dependent to the consumptions of glucose and Co ions. Its advantages are high enzyme activity and simple process. The said product can be used for preparing acrylamide.

Description

Technical field: [0001] Glucose-Co 2+ The technical method for preparing nitrile hydratase by coupled supplementary fermentation belongs to the technical field of biocatalytic production of acrylamide. Background technique: [0002] Acrylamide is a widely used organic chemical raw material. There are no less than a hundred kinds of products synthesized from it, among which polyacrylamide is the most widely used. Nitrile hydratase (NHase) is a catalyst for biocatalytic production of acrylamide, which can catalyze the hydration reaction of acrylonitrile to generate acrylamide. Acrylamide production process see figure 1 , the reaction formula is as follows: <chemistry num="001"> <chem file="03121944_cml001.xml" / > < / chemistry> [0003] In industrial production, improving the fermentation activity of nitrile hydratase can make the fermentation broth have a higher catalytic ability per unit volume, and can increase the production capacity of acrylamide wit...

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Application Information

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IPC IPC(8): C07C231/06C07K14/355C12N1/20C12N9/14C12N9/78C12N9/88C12P13/02
Inventor 李春刘铭曹竹安
Owner TSINGHUA UNIV
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