Method for positioning genes related to body odor
A positioning method and gene technology, applied in the fields of genetics, clinical medicine, dermatology, genomics, and molecular biology, can solve the problems of inability to make further progress in cloning and positioning, and inability to get out.
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Embodiment 1
[0073] Taking the positional cloning method as an example to locate the bromhidrosis-related genes, a large sample family of 46 people (1 / 3 of which are bromhidrosis patients) in 4 generations was used, and 400 STR primers were used to scan and genotype 22 autosomes and X sex chromosomes. Using GENEHUNTER software for two-point and multi-point linkage analysis, the gene loci related to underarm odor in the population were located in 11 regions of chromosomes 1, 2, 3, 6, 7, 11, and 14. On this basis, For 497 members of 112 underarm odor core families (among them, 352 underarm odor patients constituted 401 pairs of affected siblings), 11 pairs of primers were used to scan two regions of chromosome 2 in encryption. The scanning density was reduced from the original 10cM to 5cM, and the GENEHUNTER software was used for parametric and non-parametric linkage analysis and TDT test. The results showed that D2S163 and D2S2354 were closely linked with bromhidrosis-related genes, and then...
Embodiment 2
[0076] Take correlation analysis (or linkage disequilibrium analysis) as an example to locate bromhidrosis related genes. Taking 112 unrelated patients with underarm odor and 112 normal people (without underarm odor) as the research objects, the HLA gene locus located on chromosome 6 and the ABO locus on chromosome 9 were analyzed using serological methods and molecular biology. Allelic polymorphism analysis was carried out by scientific method, and the allele frequency of each locus was compared between the two groups. It was found that at the two gene loci, there was no significant difference in the allele frequency between the underarm odor group and the normal group, indicating that the two groups There is no correlation between one point and underarm odor.
Embodiment 3
[0078] Take underarm odor-related genes, or related diseases or symptoms as an example.
[0079](1) Because 99% of the underarm odor patients with familial aggregation are accompanied by greasy cerumen embolism (oily ear) and passed on to the parents at the same time, it shows that there is a certain chain relationship between the two, both of which are glandular tissues, The former is apocrine glands, and the latter is cerumen glands, suggesting a certain relationship between the two glands;
[0080] (2) Because underarm odor is mainly caused by substances such as fatty acids in the secretion of apocrine sweat glands, the onset of the odor is regulated by the gonads and endocrine system, so the related genes involved in underarm odor include: genes that control or regulate the secretion of apocrine glands or loci, adrenergic-related genes or loci, endocrine system-related genes or loci;
[0081] (3) Since the secretion of apocrine glands contains lipids, the genes involved m...
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