A general simulation system of a measurement and control subsystem of a minisatellite spread spectrum system comprises a measurement and control signal processing simulation module, a measurement and control interface simulation module, a GPS simulation module and a simulation control platform. The general simulation system can achieve radio frequency processing, intermediate frequency processing and baseband processing of measurement and control signals, achieve simulation with physical interfaces of other lower computers, and achieve output or forwarding of GPS pulse per second signals and broadcasting of time and orbital data when being in a semi-physical working mode, wherein the physical interfaces comprises the CAN bus interface, the remote measurement and control interface, the direct instruction interface, the clock output interface and the star arrow separation signal output interface. The general simulation system can receive remote measurement and control data in an LAN bus for interface simulation and GPS simulation when being in a full-digital working mode. Configurable function board cards are selected, the PXI bus technology is adopted, the integration degree is high, configuration is flexible, cost is low, function extension of the system can be conveniently conducted, the requirement for verification of multiple new technologies of the minisatellite measurement and control subsystem can be met, and the electric performance test environment of the measurement and control subsystem can be provided for development of a novel minisatellite platform.