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Method for preparing alpha-fetoglobulin transspecific factor

A technology of alpha-fetoprotein and transfer factor, which is applied in the biological field to achieve the effect of strong immune regulation function

Inactive Publication Date: 2003-12-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, non-specific transfer factor and hepatitis B specific transfer factor have been applied clinically, with clear curative effect and no obvious adverse reactions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 A preparation method of alpha-fetoprotein-specific transfer factor of the present invention 1. Preparation of immune antigen:

[0025] (1) Preparation of complete Freund's antigen: grind 15g of lanolin and 85ml of liquid paraffin oil until homogeneous, then pressurize for 30', take 1ml of the above-mentioned incomplete adjuvant + 1mg of BCG to prepare Freund's complete antigen.

[0026] (2) Preparation of immune antigen: Take 1ml of alpha-fetoprotein (including 20ug) and add 1ml of Freund's complete adjuvant. First, put the adjuvant in a sterile agate mortar, and add the antigen dropwise while grinding until it does not disperse on the water surface. , forming a water-in-oil formulation. 2. Immunization procedure:

[0027] Get healthy pigs (about 50 kilograms).

[0028] (1) The first immunization: 2ml of the antigen was injected subcutaneously at pig limbs, armpit, groin, country nest, etc. at multiple points, 0.2ml per point, with an interval of 10 days.

...

Embodiment 2

[0036] Example 2 Partial application of alpha-fetoprotein-specific transfer factor

[0037] 1. The alpha-fetoprotein-specific transfer factor obtained by the method of the present invention can significantly reduce the tumor size of mice bearing liver cancer and prolong the survival time of the animal model.

[0038] 2. The alpha-fetoprotein-specific transfer factor obtained by using the patented technology of this invention can be made into injection preparations and oral preparations for the treatment of primary liver cancer, which has great social and economic benefits.

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PUM

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Abstract

The preparation method of alpha-fetoprotein transspecific factor includes the following steps: using full length or partial fragment of alpha-fetoprotein extracted from embryonic liver or expressed by gene engineering to immunize animal, and collecting the transspecific factor obtained by separating spleen and leukocyte of immunized animal. The alpha-fetoprotein transspecific factor prepared with said method has strong immunoregulation function, and the preparation made up by using said transspecific factor can be used for curing primary liver cancer and making auxiliary therapy of liver cancer. It can be made into the forms of injection and oral liquor.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a preparation method of transfer factor, in particular to a preparation method of alpha-fetoprotein-specific transfer factor. The prepared preparation can be used in the treatment of primary liver cancer and adjuvant therapy of tumors. Background technique [0002] Primary liver cancer is a common malignant tumor that endangers human health. Epidemiological survey found that primary liver cancer is closely related to chronic infection of HBV and HCV. my country is a high-incidence area of ​​viral hepatitis. At present, there are more than 100 million HBV and HCV infected people, which leads to a high incidence of liver cancer. According to statistics from the Ministry of Health, primary liver cancer has been the second leading cause of death among all tumors since the 1990s. Although the efforts of many oncology experts have achieved a variety of treatment methods such as sur...

Claims

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Application Information

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IPC IPC(8): A61K39/395A61P35/00C07K16/18
Inventor 陈智朱海红周林福
Owner ZHEJIANG UNIV