Method for establishing fingerprint of red-rooted salvia root fat-soluble component and its standard map

A technology of fingerprints and establishment methods, which is applied in the establishment of fingerprints of the fat-soluble components of Salvia Miltiorrhiza and its standard spectra, achieving good reproducibility, high precision, and the effect of preventing myocardial infarction

Inactive Publication Date: 2004-01-28
TIAN JIN KINSLY PHARMA R&D
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Problems solved by technology

At present, there is no report on the establishment of fingerprints of fat-soluble components in Danshen medicinal materials

Method used

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  • Method for establishing fingerprint of red-rooted salvia root fat-soluble component and its standard map
  • Method for establishing fingerprint of red-rooted salvia root fat-soluble component and its standard map
  • Method for establishing fingerprint of red-rooted salvia root fat-soluble component and its standard map

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Experimental program
Comparison scheme
Effect test

Embodiment 11

[0026] Embodiment 11. Apparatus and reagent 1.1 Apparatus

[0027] Agilent 1100 high performance liquid chromatograph; Agilent VWD ultraviolet variable wavelength detector; Aotai chromatography star workstation. 1.2 Reagent

[0028] Tanshinone IIA (for content determination) was purchased from China National Institute for the Control of Pharmaceutical and Biological Products; methanol, chromatographically pure; self-made distilled water; syringe-type microporous membrane filter, organic system, diameter 13mm, 0.45μm; Salvia miltiorrhiza produced in the medicinal material base, commercially available salvia miltiorrhiza, purchased from Tianjin medicinal material company. 2. Methods and results 2.1 Chromatographic conditions

[0029] Chromatographic column: Octadecyl bonded silica gel (4.6×250mm, 5μ) American Aotai Company; mobile phase: methanol: water 3:1; detection wavelength: 270nm; flow rate: 1ml / min; column temperature: 40°C; The number of theoretical plates is calculat...

Embodiment 2

[0048] Preparation of the test solution Take 0.3g of Danshen powder, add 50ml of methanol, sonicate for 25min, let cool to room temperature, filter, and obtain. As the test drug, 10 batches of Salvia Miltiorrhiza produced in Tasly Shangluo Medicinal Material Base were used. Other conditions are with embodiment 1.

[0049] Results: The statistical results of the relative retention time and relative peak area of ​​the common peaks of ten batches of medicinal materials are shown in Table 3 and Table 4 below. Table 3: Statistical batch number\total of relative retention time of 10 batches of Salvia miltiorrhiza

[0050] 1 2 3 4 5 6 7有指纹峰材1104 0.347 0.389 0.445 0.568 0.678 0.815 1材1105 0.351 0.380 0.442 0.566 0.683 0.813 1材1106 0.355 0.387 0.441 0.561 0.682 0.809 1材1107 0.346 0.375 0.443 0.563 0.680 0.806 1材1108 0.346 0.374 0.444 0.565 0....

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Abstract

The solution of sample is prepared according to following steps. Powder of red sange root 0.3 g is fetched with methanol 50ml being added. Then, ultrasound operation is carried out for the solution just prepared. Cooling the preparing solution to room temperature and shaking up obtains the solution of sample. Reference substance is prepared according to following steps. fetching an amount of tanshinone IIA with methanol being added produces the solution of reference substance (20 ª–g/ml). The standard finger print of fat soluble component of red sange root is obtained through measurement and calculation by using high performance liquid chromatograph for 5 ª–L of sample solution and 5 ª–L of reference substance. The method provides the advantages of simple, stable, high accuracy and good repeatability. Thus, the invention can be used to control quality and distinguish whether the product is true or false.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of traditional Chinese medicinal materials. Specifically, the present invention relates to a method for establishing fingerprints of fat-soluble components of Salvia miltiorrhiza, and also relates to an HPLC fingerprint of fat-soluble components of Salvia miltiorrhiza obtained by the method. Background technique [0002] Salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge. It is produced in most parts of the country. However, due to the harvesting season, place of origin and harvesting period, etc., it is easy to cause uneven quality, and the quality is difficult to guarantee. At present, regarding the quality requirements of Danshen medicinal materials and their compound preparations, only one or two active components of Danshen are often selected as indicators for quantitative determination and qualitative identification. Tianjin Jin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/36G01N30/40
Inventor 张文生黄芝娟
Owner TIAN JIN KINSLY PHARMA R&D
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