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Polyninylalcohol degradative enzyme high productive bacteria and method of its seed selection and uses said bacteria to produce polyving/alcoho/ degradation enzyme by fermentation method

A polyvinyl alcohol and enzyme-degrading technology, applied in biochemical equipment and methods, enzymes, enzymes, etc., can solve the problem of no Penicillium fungus degrading PVA and producing enzymes.

Inactive Publication Date: 2004-07-21
JIANGNAN UNIV
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  • Abstract
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Problems solved by technology

[0004] At present, the strains with PVA-degrading ability reported at home and abroad are all bacteria or symbiotic bacteria, and there is no report of Penicillium fungi degrading PVA and producing enzymes

Method used

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  • Polyninylalcohol degradative enzyme high productive bacteria and method of its seed selection and uses said bacteria to produce polyving/alcoho/ degradation enzyme by fermentation method
  • Polyninylalcohol degradative enzyme high productive bacteria and method of its seed selection and uses said bacteria to produce polyving/alcoho/ degradation enzyme by fermentation method
  • Polyninylalcohol degradative enzyme high productive bacteria and method of its seed selection and uses said bacteria to produce polyving/alcoho/ degradation enzyme by fermentation method

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Embodiment Construction

[0084] Use Penicillium CCTCC NO: M 203035 to prepare PVA degrading enzyme.

[0085] Fermentation medium (g / L): Glucose 5, PVA 40, NH 4 Cl8, KH 2 PO 4 3, yeast paste 1, MgSO 4 0.05, CaCl 2 0.05, FeSO 4 ·7H 2 O 0.02, NaCl 0.02, dilute to 1L with deionized water, pH6.0;

[0086] Fermentation conditions: shake flask culture on a rotary shaker, the rotation speed is 200r / min, and the culture temperature is 30°C. Get the slant strains and make spore suspension into a 500ml Erlenmeyer flask equipped with 150ml seed culture medium, and cultivate for 40 hours. The cultured 5ml seed solution was inserted into a 250ml Erlenmeyer flask containing 30ml of fermentation medium, fermented to produce enzymes for 72 hours, and the initial pH was controlled at 6.4.

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Abstract

A Penicillium sp. (CCTCC No.M203035) for generating the degradative enzyme of PVA with high output is selectively cultured from the textile sewage through shake-flask culture. Its advantage is high and stable activity to degradate PVA.

Description

technical field [0001] A polyvinyl alcohol-degrading enzyme high-yield bacterium, its breeding method, and the production of polyvinyl alcohol-degrading enzyme by fermentation of the bacterium belong to the technical field of bioengineering, in particular, it belongs to the direct processing of polyvinyl alcohol slurry on the surface of fabrics by using biological enzymes. The technical field of degradation. Background technique [0002] As a sizing agent in the textile industry, polyvinyl alcohol (PVA) occupies a dominant position in all kinds of polymer synthetic sizing agents due to its excellent characteristics such as stable viscosity, pH, miscibility and emulsifying ability, and its consumption has reached 40%. However, in the desizing process of PVA size, traditional alkali desizing and oxidant desizing have always had the disadvantages of damaging fabric surface fibers and high costs, and due to the refractory nature of PVA itself, it is difficult to desize wastewat...

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Application Information

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IPC IPC(8): C12N1/14C12N9/00D06M16/00
Inventor 陈坚钱鼎堵国成李寅伦世仪
Owner JIANGNAN UNIV
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