Polyninylalcohol degradative enzyme high productive bacteria and method of its seed selection and uses said bacteria to produce polyving/alcoho/ degradation enzyme by fermentation method
A polyvinyl alcohol and enzyme-degrading technology, applied in biochemical equipment and methods, enzymes, enzymes, etc., can solve the problem of no Penicillium fungus degrading PVA and producing enzymes.
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[0084] Use Penicillium CCTCC NO: M 203035 to prepare PVA degrading enzyme.
[0085] Fermentation medium (g / L): Glucose 5, PVA 40, NH 4 Cl8, KH 2 PO 4 3, yeast paste 1, MgSO 4 0.05, CaCl 2 0.05, FeSO 4 ·7H 2 O 0.02, NaCl 0.02, dilute to 1L with deionized water, pH6.0;
[0086] Fermentation conditions: shake flask culture on a rotary shaker, the rotation speed is 200r / min, and the culture temperature is 30°C. Get the slant strains and make spore suspension into a 500ml Erlenmeyer flask equipped with 150ml seed culture medium, and cultivate for 40 hours. The cultured 5ml seed solution was inserted into a 250ml Erlenmeyer flask containing 30ml of fermentation medium, fermented to produce enzymes for 72 hours, and the initial pH was controlled at 6.4.
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