The invention provides a high-efficiency compound enzyme preparation used for a pretreatment process of textile fabrics, particularly cotton fabrics, and application thereof. The compound enzyme preparation mainly comprises the following components by final concentration: 50 to 100g/L of polysiloxane, 50 to 100g/L of fatty alcohol-polyoxyethylene ether, 80 to 150g/L of Tween 20 to 80 series, 80 to 150g/L of Span 20 to 80 series, 30 to 80g/L of sorbitol, 3 to 10g/L of calcium chloride, 5 to 15g/L of sodium benzoate, 800 to 1,000U/mL of alkaline pectinase, 1,000 to 1,200U/nL of cellulase, and water serving as a solvent. The compound enzyme preparation is applied to the pretreatment of the textile fabrics to replace the conventional alkali boiling off process, can effectively reduce production cost, reduce labor intensity and enable the quality of the product to be obviously improved, can also reduce the salt content and BOD, COD and the like in the water discharged into the environment and obviously reduce the pollution to the environment, and is a green process route which accords with the sustainable development.