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Method for raising SPE hamster for preparing various biological products

A technology of biological products and pathogens, which is applied in the fields of vehicle rescue, urns, animal husbandry, etc., and can solve the problems that the safety of biological products is not guaranteed.

Inactive Publication Date: 2004-08-04
GROVAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the safety of the above-mentioned biological products has not been guaranteed so far.

Method used

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  • Method for raising SPE hamster for preparing various biological products
  • Method for raising SPE hamster for preparing various biological products

Examples

Experimental program
Comparison scheme
Effect test

reference example 1

[0048] Microbial Contamination Experiment

[0049] 1-1) Detection of parasites

[0050] After tape-taking the specimen, examine it with a light microscope for the presence of any exogenous parasites. At the same time, to check for the presence of any endogenous parasites, the experimental animals were sacrificed, dissected, and stomach, small intestine, and large intestine tissue samples were prepared. The specimens were stained with hematoxylin and eosin and examined under a light microscope.

[0051] 1-2) Detection of bacteria

[0052] Check for the presence of bacteria based on the following three methods.

[0053] a. Culture method—for all bacteria except Helicobacter spirochetes, Mycoplasma pneumoniae, and Clostridioides.

[0054] Tracheal, sinus, and intestinal material was taken with sterile cotton swabs and spread out on culture plates for bacterial testing. Then, follow the SOP (Standard Operating Procedure, standard operating procedure) for processing.

[0...

reference example 2

[0062] Preparation of the Surgical Isolation Room

[0063] The interior of a surgical isolation room (Daehan Biolink Co. Ltd., Korea) was sterilized with alcohol and chlorhexidine gluconate solution, and then its positive pressure was maintained with active air flow. A immersion bath was filled with a 0.2% peracetic acid solution. The operating tools and other necessities are put into this isolation room for the surgical operation, and a blood agar plate (SIGMA) is prepared for immediate detection of possible microbial contamination in the specimen (freshly taken from the embryo).

[0064]

[0065] Screening of hamsters for production of SPF hamsters

[0066] 1-1) Initial screening of the hamster group

[0067] Following the method described in Reference Example 1, the golden Syrian hamsters obtained from the Chengdu Institute of Biological Products in China were subjected to microbial contamination experiments (see Table 1). As a result, a group of hamsters not conta...

experiment example 1

[0137] Safety test of Japanese hepatitis attenuated live vaccine in the present invention

[0138] In order to confirm the safety of the Japanese hepatitis live attenuated vaccine on the human body, a safety test was carried out. The Japanese hepatitis live attenuated vaccine was prepared with the PHK cells of the SPF hamster (Example 5) of the present invention.

[0139] 1-1) Detection of HPyV

[0140] In order to detect whether there is HPyV in the Japanese hepatitis attenuated live vaccine of the present invention, use ABI 7700 sequence detection system to determine whether there is HPyV specific sequence, the method is as described in Example 5-1). The results are summarized in Table 13 below.

[0141] test group

HPyV-specific sequence

negative control group

* -

Japanese hepatitis attenuated live vaccine of the present invention

-

positive control group

* +

[0142] * -: not detected

[0143] *...

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Abstract

The present invention relates to a method of producing a SPF (specific pathogen free: SPF) hamster for the preparation of a variety of biological products. More particularly, the present invention relates to a method of producing the SPF hamster for the preparation of a variety of biological products, that is free of microbiological contaminants such as parasites, bacteria, and exogenous- and endogenous viruses, etc. with the potential to cause diseases. Since the SPF hamster produced according to the method of the present invention carries no contaminants such as parasites, bacteria, and exogenous- and endogenous viruses, etc., the primary cells originated from said SPF hamster and the biological products using the same do not induce any opportunistic infection by microbial contaminants that can be present in animal cells used for existing biological products such as vaccine, etc. Therefore, the SPF hamster produced according to the present invention can be safely used for preparing a variety of biological products that are applied to a human.

Description

technical field [0001] The present invention relates to a method for producing SPF (Specific Pathogen Free: SPF) hamsters, which can be used to prepare various biological products. In particular, the present invention relates to a method for producing SPF hamsters from which various biological products prepared are not infected with potentially disease-causing microorganisms such as parasites, bacteria, and endogenous- and exogenous viruses . Background technique [0002] Vaccination is a method of conferring protective and active immunity on a human host without inducing any disease on the host, using antigens associated with infectious diseases. For example, antigens of disease-associated pathogens or the pathogen itself can suitably elicit a very specific immune response mediated by humoral immunoregulatory B lymphocytes or by cellular immune regulatory T lymphocytes, thus allowing titers of protective antibodies to The degree of increase to the same or similar to the h...

Claims

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Application Information

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IPC IPC(8): A01K67/027A01K67/02
CPCA01K67/02A61G17/08A61G2203/70
Inventor 成济庆吴承炫申善行
Owner GROVAX
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