Method for extracting glycerrhizic acid from licorice

A technology of glycyrrhizic acid and licorice, applied in the directions of steroids, organic chemistry, etc., can solve the problems of high energy consumption of glycyrrhizic acid hydrolysis and oxidative denaturation, long time consumption, and high cost, and achieve simplified operation, high extraction efficiency, and recovery rate. high effect

Inactive Publication Date: 2004-09-01
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the existing extraction technology time-consuming; the concentration process consumes a lot of energy and will cause the hydrolysis and oxidative denaturation of glycyrrhizic acid; and the defects of high cost, thereby providing an organic combination of microwave-assisted extraction and solvent extraction , using a low water-soluble organic solvent as the extraction agent, using solvent extraction to effectively couple concentration and purification, and directly purifying glycyrrhizic acid from the extract, so that the entire glycyrrhizic acid purification process is fast, efficient and simplified

Method used

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  • Method for extracting glycerrhizic acid from licorice
  • Method for extracting glycerrhizic acid from licorice
  • Method for extracting glycerrhizic acid from licorice

Examples

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Effect test

Embodiment 1

[0022] Embodiment 1. extract glycyrrhizic acid from Radix Glycyrrhizae

[0023] After the fresh licorice with a diameter of 0.5-1.5 cm was air-dried, it was processed into licorice samples of different particle sizes. Weigh 5 grams of licorice sample and place in a flask, add 100ml of water to mix, put into microwave-assisted extraction equipment, microwave heating power is 700W, carry out microwave interval heating for 4 minutes, filter while hot, and obtain glycyrrhizic acid extract. After cooling, fix the solution, adopt HPLC method to analyze the content of glycyrrhizic acid in the extract, and calculate the recovery rate of glycyrrhizic acid according to formula (1) to be 80.3%.

[0024]

[0025] 100ml of the glycyrrhizic acid extract and 20ml of petroleum ether containing 10% TRPO were added respectively to a beaker and magnetically stirred. Add sulfuric acid while stirring to adjust the pH value of the mixture to 3.0, then transfer the mixture to a centrifuge tube, ...

Embodiment 2

[0032] Embodiment 2. extract glycyrrhizic acid from Radix Glycyrrhizae

[0033] After the fresh licorice with a diameter of 0.5-1.5 cm was air-dried, it was processed into licorice samples of different particle sizes. Weigh 5 grams of licorice sample and place in a flask, add 100ml of water to mix, put into microwave-assisted extraction equipment, microwave heating power is 500W, carry out microwave interval heating for 6 minutes, filter while hot, and obtain glycyrrhizic acid extract. After cooling, the solution was fixed, and the HPLC method was used to analyze the content of glycyrrhizic acid in the extract, and the recovery rate of glycyrrhizic acid calculated by formula (1) was 70%.

[0034] 100ml of this glycyrrhizic acid extract and 100ml of TBP petroleum ether containing 50% were added into a beaker respectively, and magnetically stirred. While stirring, add hydrochloric acid to adjust the pH value of the mixture to 3.0, then transfer the mixture to a centrifuge tube,...

Embodiment 3

[0038] Embodiment 3. extract glycyrrhizic acid from Radix Glycyrrhizae

[0039] After the fresh licorice with a diameter of 0.5-1.5 cm was air-dried, it was processed into licorice samples of different particle sizes. Weigh 5 grams of licorice sample and place it in a flask, add 100ml of water to mix, put into microwave-assisted extraction equipment, microwave heating power is 200W, carry out microwave interval heating for 10 minutes, filter while hot, and obtain glycyrrhizic acid extract. After cooling, the solution was fixed, and the HPLC method was used to analyze the content of glycyrrhizic acid in the extract, and the recovery rate of glycyrrhizic acid calculated by formula (1) was 65%.

[0040] 100ml of the glycyrrhizic acid extract and 50ml of n-hexanol were added to the beaker respectively, and magnetically stirred. While stirring, add hydrochloric acid to adjust the pH value of the mixture to 2.5, then transfer the mixture to a centrifuge tube, centrifuge for 3 min, ...

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Abstract

specifically a process for extracting glycyrrhizic acid from licorice roots, wherein the glycyrrhizic acid is extracted from the licorice root rapidly and effectively by using the method of microwave auxiliary extraction, and then organic extracting agent, e.g. tributyl phosphate, trialkyl phosphine oxide, isooctanol, n-hexyl alcohol, isopentyl alcohol are used for solvent extraction, after stewing or centrifugal phase-splitting, the glycyrrhizic acid will be enriched in organic phase, by using water as solution, and regulating the pH of the miscible liquids to 6.0-12.0, the inverse extraction for glycyrrhizic acid can be carried out, thus obtaining the aqueous solution of the enriched glycyrrhizic acid, and the refined glycyrrhizic acid is obtained through decoloration, acidification, stewing, centrifugal deposition and drying.

Description

technical field [0001] The invention relates to a method for extracting glycyrrhizic acid from licorice. Background technique [0002] Glycyrrhizic acid (Glycyrrhizic Acid, GA) is the earliest saponin component isolated from licorice. It consists of glycyrrhetinic acid and two molecules of glucuronic acid. Its structure is: [0003] R = 2 molecules of glucuronic acid [0004] Bibliography (the in-depth development and comprehensive utilization of Radix Glycyrrhizae in medicine etc., Hu Jinfeng, Shen Fengjia, Chinese herbal medicine, 26,39-44,1995) the pharmacological effect of glycyrrhizic acid is mainly manifested in: a) has the ability to induce interferon ( IFN) role. b) Hypolipidemic effect: reduce blood cholesterol content and treat atherosclerosis. c) Anti-HIV. In addition, glycyrrhizic acid is also widely used in industries such as food and cosmetics. [0005] The traditional leaching process generally adopts the method of heat reflux or decocting with wate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 牛国光谢渝春刘会洲
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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