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Nano-liter spraying device

A technology of nano-liter spray and spray head, applied in the field of mass spectrometry, which can solve the problems of unsatisfactory spray stability, short service life and difficult operation of the spray head, and achieve the effect of simple production, convenient operation and easy spraying

Inactive Publication Date: 2004-12-29
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem is: the metal coating is easy to fall off under the condition of high voltage discharge, so the service life of the nozzle is short
But because the thin metal wire is very soft, it is very difficult to operate, and the spray stability of the nozzle is not ideal.

Method used

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  • Nano-liter spraying device
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Embodiment Construction

[0016] The present invention is further described by the following examples, but not limited to the examples.

[0017] according to figure 2 As shown, the main body of the nanoliter spray device is a three-way, made of stainless steel, and the glass spray head 1 is drawn from a glass capillary of Φ1.4 on a multifunctional glass microelectrode controller. Finished spray head tip is in closed state, is 36mm, and injection needle 7 and 12 all use No. 7 medical needles (also available No. 6 or No. 8). The diameter of the molybdenum wire 2 is 30-100 μm (50 μm diameter is usually available), and the length is 18 mm. One end of the molybdenum wire 2 is mechanically welded on the tip of the needle 7, and then passes from one end of the stainless steel tee 5 to the other end; the glass spray head 1 The port of the tee 5 is sealed and connected through the silicone rubber ring 3 and the nut 4, and the molybdenum wire 2 is inserted therein; the third port of the tee 5 is the gas passag...

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Abstract

The Nasheng sprayer for mass spectrographic analysis consists of one tee junction, one spraying head, one injection needle and molybdenum filament. The spraying head is connected with one end of the tee junction hermetically, the molybdenum filament is welded to the tip of the needle, the needle is inserted into the other end of the tee junction, the front end of the molybdenum filament is stretched into the tip of the spraying head, and the third port of the tee junction is used as air passage. The molybdenum filament provides electric contact and the air passage provides auxiliary air for assisting spraying. The sprayer has convenient making, simple operation, high spraying stability and wide application foreground.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry, and in particular relates to a nanoliter spray device. Background technique [0002] Electrospray ionization is a mass spectrometer soft ionization technique. It can be effectively combined with liquid chromatography (liquid chromatography) and capillary electrophoresis (capillary electrophoresis). Electrospray mass spectrometry (ESI-MS) and its coupled techniques have been widely used in the analysis of biological macromolecules, especially in the molecular weight determination and sequence analysis of proteins in proteomics. [0003] Nano-electrospray technology is a new technology of mass spectrometry developed in recent years. Compared with conventional electrospray, nano-electrospray has higher sensitivity and less sample consumption. The key to nano-electrospray technology is to provide electrical contact on the nano-spray tip. The main methods are: electroplating metal (mainl...

Claims

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Application Information

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IPC IPC(8): B05B5/025G01N30/96
Inventor 翟建军水雯箐包慧敏许雪姣陈先杨芃原
Owner FUDAN UNIV
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