Pouring type biologic reactor system in vitro constructure of dual active skin

A bioreactor, perfusion technology, applied in microorganisms, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of inability to simulate the growth environment, difficult to enlarge, complex structure, etc., to achieve a good in vivo growth environment, easy to enlarge, The effect of increasing the area

Inactive Publication Date: 2005-01-05
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The technical problem to be solved in the present invention is to disclose a perfusion bioreactor system constructed in vitro with double-layer active skin, so as to overcome the shortco...

Method used

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  • Pouring type biologic reactor system in vitro constructure of dual active skin
  • Pouring type biologic reactor system in vitro constructure of dual active skin

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Experimental program
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Embodiment Construction

[0020] see figure 1 and figure 2 , the perfusion bioreactor system constructed in vitro with double-layer active skin of the present invention at least includes: a liquid addition device, a perfusion bioreactor, a liquid collection device, a liquid return device and an air supply device;

[0021] Said perfusion bioreactor comprises: a closed container 2 provided with a loam cake 1; a porous support plate 5 arranged in the closed container 2 to separate the closed container 2 into an upper chamber 3 and a lower chamber 4, arranged on the porous support plate 5 above the fastening seal 6 close to the inner wall of the closed container 2; the scaffold material or cell material composite 7 for skin construction is placed flat on the porous support plate 5 and fixed by the fastening seal 6, and the epidermis is porous when it is constructed The support plate 5 can provide a gas-liquid interface; the upper chamber 3 is provided with a liquid inlet 8 and a liquid outlet 9, the lowe...

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Abstract

The invention is a double-layer active skin-composed in vitro pouring bioreactor system, including a liquid-adding device, a pouring bioreactor, a liquid-collecting device, a liquid reflux and a gas supply device; the liquid-adding device is connected with a liquid inlet of the pouring bioreactor, the liquid-collecting device is connected with a liquid outlet of the pouring bioreactor to collect the culture medium after culture, the liquid reflux is connected with the liquid -adding and -collecting devices through pipelines, and the gas supply device is connected with a gas inlet of the pouring bioreactor through pipeline; the bracket or cell material compound for composing the skin is smoothed out on a multiporous supporting plate of the pouring bioreactor. It has simple structure, easy to enlarge and can make wet-hot sterilization, maintain axenic environment for a long time and primely simulate in vivo growth environment, implementing the course from vaccinating to derma composing to the entire double-layer active skin composing in vitro by pidermis.

Description

technical field [0001] The invention relates to a bioreactor, in particular to a perfusion bioreactor for constructing double-layer active skin in vitro in medicine and tissue engineering. Background technique [0002] Skin is the largest tissue in the human body, with a surface area of ​​about 1.5-2.0m 2 . As the outermost barrier of the human body, the skin is also the most vulnerable tissue. Approximately 60,000-80,000 burn patients require skin grafts each year. [0003] Currently used autologous skin grafts, allogeneic skin grafts, and xenogeneic skin grafts have problems such as insufficient donor sites, immune rejection, and disease transmission. The emergence of tissue-engineered skin has injected new vitality into skin transplantation. [0004] The research on tissue-engineered skin began in the 1970s. So far, various skin substitutes with clinical value have been developed, which can be divided into epidermal substitutes, dermal substitutes and composite skin s...

Claims

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Application Information

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IPC IPC(8): A61L27/60C12M3/00C12N5/08
CPCC12M23/58C12M29/10C12M41/26C12M41/32
Inventor 谭文松周燕丁春梅赵一鸣顾小华
Owner EAST CHINA UNIV OF SCI & TECH
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