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Electrochemical detection method and device of integrated in chip capillary electrophoresis

A technology of capillary electrophoresis and detection method, which is used in measurement devices, scientific instruments, material analysis by electromagnetic means, etc., can solve the problems of narrow application range, low sensitivity, easy contamination of working electrodes, etc., and achieve high-sensitivity detection. , to ensure reproducible, convenient and effective monitoring of the effect

Inactive Publication Date: 2005-01-12
NANJING UNIV
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Problems solved by technology

[0004] However, the three electrochemical detection methods currently developed have their own advantages and disadvantages.
The amperometric method can detect electroactive substances with relatively high sensitivity, but the working electrode is easily contaminated; the conductometric method can detect substances that affect the conductivity of the solution, which has good versatility, but the sensitivity is not high; the potentiometric method can detect specific substances. The selectivity is relatively high, but the scope of application is relatively narrow
Another critical issue is the precise positioning of the working electrode, which has become a common problem due to the miniaturization of the tubing

Method used

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  • Electrochemical detection method and device of integrated in chip capillary electrophoresis
  • Electrochemical detection method and device of integrated in chip capillary electrophoresis
  • Electrochemical detection method and device of integrated in chip capillary electrophoresis

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with drawings and embodiments.

[0022] The water used in the experiment process is double distilled water, and the samples for separation and detection are all pure substances. The stock solutions of lithium chloride, sodium chloride, potassium chloride, dopamine, and epinephrine were all prepared to a concentration of 10 mM with twice distilled water, and diluted to the required concentration with separated running buffer before use. Phosphate buffer can be used for the separation and detection of lithium chloride, sodium chloride and potassium chloride, and Tris buffer can also be used; phosphate buffer can be used for the separation and detection of dopamine and adrenaline.

[0023] exist figure 1 In the chip capillary electrophoresis integrated electrochemical system shown, the chip (1) is cleaned and dried before the experiment, and then fixed on the chip support platform (5) with double-sided a...

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Abstract

The method includes following steps: using high voltage power supply dedicated to capillary in chip monitors whether separator tube and sample adding tube are unblocked; high voltage is applied to separator tube for a longer time to activate working electrode; if tubes are unblocked and electrode is activated, electrode holder is clamped on optical fiber clip matching to 3D adjuster; under stereomicroscope, front end of working electrode is put into micro tube; working electrode made from ultramicro disc carbon fiber is put inside micro tube through fine 3D adjuster. The invented device can detect electric active substance as well as non-electric active substance. Separating and detecting nonorganic as well as organic sample are realized through controlling electric potential on specific position and position of working electrode.

Description

technical field [0001] The present invention is a new type of electrochemical detection technology and special device integrated in chip capillary electrophoresis. Specifically, in the process of integrating electrochemical technology and chip capillary electrophoresis separation technology, by precisely controlling the position of the electrochemical working electrode, High-efficiency separation and high-sensitivity detection of samples. Background technique [0002] One of the hot spots in the research frontier of analytical science is the micro-total analysis system. Traditional analytical instruments and methods require a lot of manpower and material resources, the automation of the whole process is very low, and the consumption of reagents and energy is relatively large, which will adversely affect the development of analytical science in the future. Under this background, the research of micro-total analysis system came into being. The micro-total analysis system can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 陈洪渊徐静娟鲍宁夏兴华
Owner NANJING UNIV
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