Gene chip surface processing method

A processing method and gene chip technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as inability to distinguish non-intentional, complicated surface treatment process, complicated operation process, etc., to improve immobilization efficiency, The effect of low toxicity and simple operation process

Inactive Publication Date: 2005-02-23
GENETIC ENG RES INSTITUTION SOUTHERN MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

In chip production, if the unbound free active groups on the chip surface after DNA immobilization are not blocked (or inactivated), they will combine with the DNA in the labeled sample during the hybridization process, resulting in indistinguishable non-specific hybridization signals. Causes a large increase in background and also reduces hybridization efficiency
[0007] 4) Operation process and cost
Generally, the surface treatment process is complicated by the method of covalent bonding, especially in the preparation process of multi-aminated glass slides, the operation process is complicated, time-consuming, reagents and high cost

Method used

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  • Gene chip surface processing method
  • Gene chip surface processing method
  • Gene chip surface processing method

Examples

Experimental program
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Embodiment Construction

[0028] 1) Cleaning and silanization of slides (pretreatment of slides):

[0029] Cleaning of glass slides: Soak the slides in 1M NaOH for 2 hours, shake them on a shaker during soaking, wash them with sterilized water, soak them in 5% dilute HCL overnight, and then wash them with sterilized water.

[0030] Silanization of glass slides: put the slides into a silanization solution (the silanization solution is obtained by dissolving 9% aminopropyltrimethoxysilane (APS) in methanol) for silanization for 30 minutes, then wash with 95% ethanol solution, Then wash with sterilized water 3 times and dry.

[0031] 2) Surface chemical reaction (polyacrylamide derivative polymer coating and surface activation):

[0032] Dissolve 1.2% acrylic acid and 0.8% methylene acrylamide in distilled water, and heat at 70°C for 30mins, then add 0.6% ammonium persulfate solution, mix well, and store the solution at 4°C for use.

[0033] Add an equal volume (1:1) of activation solution components to...

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Abstract

A gene chip surface processing method includes: pretreating slide by siliconizing reagent, acrylic acid and acrylic acid monomer polymerizing on slide surface by polymer polyreacting, forming covalent connecting, inducing active molecule when polyreacting, chemical reacting with amide group of polymer, and forming amino-sensitive chemical surface. It achieves low cost, simple process, and good stability.

Description

Technical field [0001] The invention relates to a method for treating the surface of a gene chip. Background technique [0002] The first problem to be solved in the preparation of gene chips is to deposit and combine DNA fragments and oligonucleotide probes on the surface of solid phase carriers, that is, the immobilization of nucleic acids. This immobilization or binding should not only be precise and reproducible, but also ensure the stable existence of nucleic acid or ligand during the ligation reaction and subsequent analysis. Therefore, whether various nucleic acid probes can be specifically, rapidly and stably immobilized on the surface of the carrier will directly affect the efficiency of the microarray. [0003] Currently, chip carrier materials are mainly glass slides. However, due to the planar structure of the glass slide and the small amount of sample loaded, the detection sensitivity is low. In order to overcome this shortcoming, researchers at home and abro...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 马文丽郑文岭吴清华
Owner GENETIC ENG RES INSTITUTION SOUTHERN MEDICAL UNIV
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