Flow injection chemiluminescent immunity detecting pool for silicane cross-linked chitosan film base and process for preparing same

Abstract

It is flow injection chemical radiation immune detection pool of silane cross-linking polyose film group which is the following: to combine the sensor and the chemical radiation flow pool to make flow injection chemical radiation immune measurement pool; the chemical radiation flow pool comprises the upper plastic board, down transparent board and silicon rubber cushion ring; to open two paths in the upper plastic board as the entrance and exit of the flow transportation; to etch a groove in its inside to embed immune sensor; the down board is glass or organic glass board with good transparency and directly lay them on the down end of electron-multiplier phototube; to adopt silicon rubber cushion ring in the middle as reaction pool where the immune reaction and radiation measurement happens; the immune sensor structure is to process chitose on the silane load surface and fix the antigen molecule and form the new immune sensor.

Description

technical field [0001] The invention relates to a flow injection chemiluminescence immunoassay analysis method, in particular to a silane cross-linked chitosan membrane-based flow injection chemiluminescence immunoassay cell and a preparation method. Background technique [0002] In recent decades, immunoassay technology has combined the specific recognition reaction between antigens and antibody molecules and the sensitivity and convenience of electrochemical, spectroscopy, acoustics and other technologies, with its high selectivity for analytes such as tumor markers. And high sensitivity, it has become one of the important analytical methods in clinical, biochemical, environmental analysis and other fields. Conventional immunoassay methods mainly include: radioimmunoassay, enzyme-linked immunoassay, fluorescence immunoassay, chemiluminescence immunoassay, etc. These methods mainly label radioactive isotopes, enzyme molecules, fluorescent substa...

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Problems solved by technology

These conventional analysis methods have cumbersome operation steps, long analysis time, large sample consumption and high determination cost

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