Flow injection chemiluminescent immunity detecting pool for silicane cross-linked chitosan film base and process for preparing same

A technology of chemiluminescence immunity and chitosan membrane, which is applied in the direction of chemiluminescence/bioluminescence, analysis through chemical reaction of materials, and measurement devices, which can solve the problems of large sample consumption, cumbersome operation steps, and long analysis time, etc. problems, to achieve the effect of simplifying the operation steps, simple and flexible operation, and simplifying the analysis steps

Inactive Publication Date: 2005-03-16
JIANGSU CANCER HOSPITAL +1
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Problems solved by technology

These conventional analysis methods have cumbersome operation steps, l

Method used

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  • Flow injection chemiluminescent immunity detecting pool for silicane cross-linked chitosan film base and process for preparing same
  • Flow injection chemiluminescent immunity detecting pool for silicane cross-linked chitosan film base and process for preparing same

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Embodiment Construction

[0017] 1. Chemiluminescence flow cell

[0018] Such as figure 1 As shown, the chemiluminescent flow cell is composed of a polytetrafluoroethylene plate, a plexiglass plate and a silicone rubber gasket. Two channels are chiseled on the upper PTFE plate (45mm×30mm×8mm) as the inlet and outlet of liquid transmission; a groove (20mm×10mm×1.5mm) is engraved on the inner side, the dimensions of length and width Plus or minus 3mm, high dimension plus or minus 1mm. A glass slide modified with a functional membrane of an antigen molecule is used to embed an immunosensor ( figure 2 The lower layer is a plexiglass plate (45mm×30mm×8mm) with good light transmission, which is placed directly above the photomultiplier tube; the middle uses a silicon rubber gasket to form a reaction pool, and both immune reaction and luminescence detection occur in this circuit.

[0019] 2. Preparation of Immunosensors

[0020] (1) The antigen to be tested is dissolved in a buffer solution. The selected...

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Abstract

It is flow injection chemical radiation immune detection pool of silane cross-linking polyose film group which is the following: to combine the sensor and the chemical radiation flow pool to make flow injection chemical radiation immune measurement pool; the chemical radiation flow pool comprises the upper plastic board, down transparent board and silicon rubber cushion ring; to open two paths in the upper plastic board as the entrance and exit of the flow transportation; to etch a groove in its inside to embed immune sensor; the down board is glass or organic glass board with good transparency and directly lay them on the down end of electron-multiplier phototube; to adopt silicon rubber cushion ring in the middle as reaction pool where the immune reaction and radiation measurement happens; the immune sensor structure is to process chitose on the silane load surface and fix the antigen molecule and form the new immune sensor.

Description

technical field [0001] The invention relates to a flow injection chemiluminescence immunoassay analysis method, in particular to a silane cross-linked chitosan membrane-based flow injection chemiluminescence immunoassay cell and a preparation method. Background technique [0002] In recent decades, immunoassay technology has combined the specific recognition reaction between antigens and antibody molecules and the sensitivity and convenience of electrochemical, spectroscopy, acoustics and other technologies, with its high selectivity for analytes such as tumor markers. And high sensitivity, it has become one of the important analytical methods in clinical, biochemical, environmental analysis and other fields. Conventional immunoassay methods mainly include: radioimmunoassay, enzyme-linked immunoassay, fluorescence immunoassay, chemiluminescence immunoassay, etc. These methods mainly label radioactive isotopes, enzyme molecules, fluorescent substa...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/52G01N33/53G01N33/533G01N33/547
Inventor 严枫林洁华鞠熀先
Owner JIANGSU CANCER HOSPITAL
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