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Method for preparing Griflola frondosa proteoglycan

A technology for proteoglycan and Grifola frondosa, which is applied in the field of preparation of Grifola frondosa proteoglycan, can solve the problems of cumbersome extraction procedures, low proteoglycan extraction yield, incomplete extraction and the like, and achieves simple steps and time-consuming The effect of short and improved utilization

Inactive Publication Date: 2005-07-06
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method for preparing proteoglycan from Grifola frondosa, so as to solve the shortcomings of low proteoglycan extraction yield, cumbersome extraction process and incomplete extraction in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] ① Submerge the fruiting body of Grifola frondosa with 10 times the volume of water, and keep it warm for 2 hours at 80°C;

[0030] ② Repeat the extraction twice;

[0031] ③ After coarse filtration, the extract is ultrafiltered with a 100,000 Dalton hollow fiber ultrafiltration membrane, and the intercepted part is collected as a water-soluble proteoglycan solution;

[0032] ④ Add 10 times volume of potassium hydroxide alkali solution with a weight percentage concentration of 6% to the fruiting bodies of Grifola frondosa after water extraction, and keep warm for 12 hours at 10°C;

[0033] ⑤ Repeat the extraction once;

[0034] ⑥ After coarse filtration, the extract is ultrafiltered with a 100,000 Dalton hollow fiber ultrafiltration membrane, and the intercepted part is collected as an alkali-soluble proteoglycan solution;

[0035] ⑦Concentrate and dry the water-soluble and alkali-soluble proteoglycan solutions together to obtain dry proteoglycan powder from Grifola fro...

Embodiment 2

[0037] ① Submerge the fruiting body of Grifola frondosa with 20 times the volume of water, and keep it warm for 1 hour at 90°C;

[0038] ② Repeat the extraction 3 times;

[0039] ③ After coarse filtration, the extract is subjected to ultrafiltration with a 500,000 Dalton hollow fiber ultrafiltration membrane, and the intercepted part is collected as a water-soluble proteoglycan solution;

[0040] ④The fruiting bodies of Grifola frondosa after water extraction were added 20 times the volume of sodium hydroxide alkali solution with a concentration of 4% by weight, and kept at 20°C for 8 hours;

[0041] ⑤ Repeat the extraction 2 times;

[0042] ⑥ After coarse filtration, the extract is ultrafiltered with a 100,000 Dalton hollow fiber ultrafiltration membrane, and the intercepted part is collected as an alkali-soluble proteoglycan solution;

[0043] ⑦Concentrate and dry the water-soluble and alkali-soluble proteoglycan solutions together to obtain dry proteoglycan powder from Gr...

Embodiment 3

[0045] ① Submerge the fruiting body of Grifola frondosa with 40 times the volume of water, and keep it warm for 2 hours at 100°C;

[0046] ② Repeat the extraction once;

[0047] ③ After coarse filtration, the extract is subjected to ultrafiltration with a 500,000 Dalton hollow fiber ultrafiltration membrane, and the intercepted part is collected as a water-soluble proteoglycan solution;

[0048] ④ Add 40 times the volume of 2% sodium hydroxide alkali solution to the fruiting bodies of Grifola frondosa after water extraction, and keep warm for 4 hours at 30°C;

[0049] ⑤ Repeat the extraction 5 times;

[0050] ⑥ After coarse filtration, the extract is ultrafiltered with a 500,000 Dalton hollow fiber ultrafiltration membrane, and the intercepted part is collected as an alkali-soluble proteoglycan solution;

[0051] ⑦Concentrate and dry the water-soluble and alkali-soluble proteoglycan solutions together to obtain dry proteoglycan powder from Grifola frondosa.

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Abstract

The invention provides a preparation method of a polyporus frondosus proteoglycan. In the method, the polyporus frondosus fruiting body is extracted by hot water at first, then the polyporus frondosus fruiting body is extracted by alkaline solution. Water soluble and alkali soluble polyporus frondosus proteoglycan solutions are obtained by hollow fiber ultrafiltration and retention of the extracted solution. The polyporus frondosus proteoglycan dry powder is obtained by concentrating and drying said solutions. By the method, the yield of the proteoglycan can be improved.

Description

Technical field: [0001] The invention relates to a preparation method of edible and medicinal mycoproteoglycan, in particular to a preparation method of grifola frondosa proteoglycan. Background technique: [0002] Grifola frondosa proteoglycan has immune activity and antitumor activity. Currently available preparation methods: hot water extraction of Grifola frondosa to obtain water extract——alcohol precipitation of the water extract—free protein removal of the precipitate—dissolution of the residue after acid extraction—alkaline solution After alcohol precipitation—deproteinization—alcohol precipitation—the precipitate is proteoglycan. The extraction yield of this method is low, about 2 / 1000 to 5 / 1000; the repeated precipitation process is cumbersome; part of the alkali-soluble matter in the Grifola frondosa after water extraction also has biological activity, and has not been extracted. (Nanba, 1987; Chem. Parm. Bull 35(3) 1162-1168; The Chemical Structure of an Antitum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/37C08B37/00
Inventor 周昌艳张劲松贾薇杨焱郭倩刘艳芳
Owner SHANGHAI ACAD OF AGRI SCI
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