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Gellan gum post-extraction method

An extraction method, gellan gum technology, applied in the post-extraction field of gellan gum, can solve the problems of difficult production technology, high price, single, etc., to avoid high manufacturing cost, improve safety and reliability, and reasonable process design Effect

Active Publication Date: 2005-07-06
HEBEI HENBO BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only the American CP keclo company in the world has achieved industrial production, and its production and sales are in a monopoly position, and its production technology is difficult, so the price is expensive
Some colleges and universities in China have also tried to produce gellan gum with relatively simple production technology, but the effect has not been ideal
Patent No. 00125858.3 discloses a new production process of microbial polysaccharide gellan gum, but the above-mentioned prior art uses membrane technology to extract gellan gum in the post-extraction process, because the price of the membrane is high and cannot be regenerated , so the manufacturing cost remains high, and the prepared product is a single variety, which cannot meet different needs and other shortcomings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The post-extraction method of gellan gum includes fermentation broth pretreatment, deacylation treatment, gel treatment, and solid-liquid separation; the process conditions in each process step are:

[0021] A, fermented liquid pretreatment: fermented liquid is adjusted pH value to 4.5, the acidic protease that adds 11ppm and accounts for fermented liquid volume 11%, concentration is 10% (NH 4 ) 2 SO 4 (W / V) solution, it was heated to 58 ° C, maintained for 20h, then added 1% chloroacetic acid (W / V) maintained for 1.5h;

[0022] B. Deacylation treatment: mix the pretreated fermentation broth with water in a volume of 1:1, stir evenly, heat up to 93° C., add a mixed sodium hypochlorite solution with a volume of 0-0.05% (v / v) to oxidize the acyl group, React for 15-20 minutes to obtain gellan gum liquid;

[0023] Add 1% adsorbent (W / V) and 1.5% filter aid (W / V) to the fermented liquid mixed with water, filter and remove cell fragments and pigments to obtain clear gella...

Embodiment 2

[0027] The post-extraction method of gellan gum includes fermentation broth pretreatment, deacylation treatment, gel treatment, and solid-liquid separation; the process conditions in each process step are:

[0028] A, fermented liquid pretreatment: fermented liquid is adjusted pH value to 4.8, adds the acidic protease of 13ppm and accounts for fermented liquid volume 12%, concentration is 10% (NH 4 ) 2 SO 4 (W / V) solution, it was heated to 60 ° C, maintained for 19h, then added 1% chloroacetic acid (W / V) maintained for 2h;

[0029] B. Deacylation treatment: mix the pretreated fermentation broth with water in a volume of 1:1, stir evenly, heat up to 92° C., add a mixed volume of 0.3% (v / v) sodium hypochlorite solution to oxidize the acyl group, and react for 20 Minutes, add 1% adsorbent (W / V) and 1.5% filter aid (W / V) to the reaction solution after deacylation treatment, filter and remove bacterial cell fragments and pigments, and obtain clear gellan gum liquid , The adsorbe...

Embodiment 3

[0033] The post-extraction method of gellan gum includes fermentation broth pretreatment, deacylation treatment, gel treatment, and solid-liquid separation; the process conditions in each process step are:

[0034] A, fermented liquid pretreatment: fermented liquid is adjusted pH value to 4.2, adds the acidic protease of 14ppm and accounts for fermented liquid volume 10%, concentration is 10% (NH 4 ) 2 SO 4 (W / V) solution, it was heated to 56 ° C, maintained for 23h, then added 1% chloroacetic acid (W / V) maintained for 2h;

[0035] B. Deacylation treatment: mix the pretreated fermentation broth with water at a volume of 1:1, stir evenly, heat up to 94°C, add 0.5% (v / v) sodium hypochlorite solution to oxidize the acyl group after mixing, and react for 18 Minutes, add 1% adsorbent (W / V) and 1.5% filter aid (W / V) to the reaction solution after deacylation treatment, filter and remove bacterial cell fragments and pigments, and obtain clear gellan gum liquid , The adsorbent is a...

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Abstract

The invention relates to a posterior extraction method of Gellan gum belonging to the microbe fermentation production field. The method comprises steps of pretreatment of the fermentation liquor, deacylation, filtration, extraction of the flocculate, solid-liquid separation, drying and pulverization. The invention overcomes the shortcomings of high manufacturing cost, single variety existing in known technology. By the deacylation treatment with different added hypochlorite, Gellan gum with different acyl content can be obtained without destruction of the Gellan gum molecular structure to meet different need. The flocculant employs alkali metal or alkaline earth chloride to substitute for partial organic solvent to improve the production safe reliability.

Description

technical field [0001] The invention relates to the field of microbial fermentation production, in particular to a post-extraction method of gellan gum. Background technique [0002] Gellan gum is a kind of transparent gel prepared by aerobic fermentation of bacteria in the genus Pseudomonas elodea using modern bioengineering technology and a special refining technology. It has good thermal stability. stability. Less dosage, good taste retention, acid resistance, alkali resistance, biological enzyme resistance, and gel strength can be adjusted according to needs, widely used in food, medicine, fine chemical industry and other industries. At present, only the American CP keclo company has achieved industrial production in the world, and its production and sales are in a monopoly position, and its own production technology is difficult, so the price is expensive. Some colleges and universities in China have also tried to produce gellan gum with relatively simple production t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04
Inventor 张禹张国佩张茶刘学珍
Owner HEBEI HENBO BIO TECH
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