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Fast detection of degradation property for biological degradation materials by utilizing bacteriological hybrid bacterium group

A technology of biodegradable materials and mixed flora, which is applied in the field of biodegradable environmental material degradation performance testing and research, can solve the problems of poor repeatability and long test time, and achieve the effects of accelerated degradation speed, large capacity and good repeatability

Inactive Publication Date: 2005-09-07
NANKAI UNIV
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Problems solved by technology

The shortcomings of these detection methods are that the test time is long and the repeatability is poor, that is, the results obtained by using different environmental samples as provenances are different.

Method used

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  • Fast detection of degradation property for biological degradation materials by utilizing bacteriological hybrid bacterium group
  • Fast detection of degradation property for biological degradation materials by utilizing bacteriological hybrid bacterium group
  • Fast detection of degradation property for biological degradation materials by utilizing bacteriological hybrid bacterium group

Examples

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Embodiment 1

[0021] The use of bacterial mixed flora to treat polyhydroxybutyrate copolyester (PHB / V), cellulose, polybutylene succinate and polybutylene adipate copolymer (PBSA), polycarbonate The degradation performance of 12 kinds of materials such as ester (PPC) and polybutylene succinate (PBS) were tested.

[0022] 1. Test equipment

[0023] 1.1 Bioreactor

[0024] In this experiment, a 1-liter Erlenmeyer flask was used as a bioreactor, and a rubber stopper was used to block the mouth of the bottle. Two holes were left on the rubber stopper, which were the air inlet and the air outlet. The connecting pipes must not leak carbon dioxide. The bioreactor is placed in a constant temperature water bath device with magnetic stirring function to ensure that the temperature is constant during the reaction process, and the detection solution is always stirred throughout the reaction process, so as to ensure that the dissolved oxygen in the mineral salt detection solution can meet the requirem...

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Abstract

The invention belongs to the field of bioenvironment material degradation property research, which comprises: separating from the environment and getting the degraded bacterium of every degradation materials, sorting and attesting and forming the mixing bacterium group, designing the device to measure the discharge of the carbon dioxide in the degradation process. Said invention measuring the degradation ability according to the principle of the mixing bacterium group can measure the carbon dioxide transformed from organic carbon in the high polymer, and because using the mixing bacterium group, the result has a good repeatability and can be used widely.

Description

technical field [0001] The invention belongs to the research field of biodegradable environmental material degradation performance detection Background technique [0002] Traditional plastics cannot be degraded in the natural environment, causing serious "white pollution". Therefore, all countries in the world attach great importance to the research and development of biodegradable materials. In recent years, many new biodegradable materials have been prepared by chemical synthesis or microbial fermentation. During the development and production of degradable materials, the detection of degradability is very important. However, there is no convenient, fast and reproducible detection method at present, so it is urgent to establish a practical method for detecting the degradation performance of biodegradable materials. [0003] Some researches have been done internationally on the detection methods of the degradation performance of biodegradable materials, and corresponding ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02G01N33/00G01N35/00
Inventor 宋存江杨超白云鹏张斌刘静陶健曾猛郑承刚金映红邓飞高文柏松
Owner NANKAI UNIV
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