Highly effective revulsion induction method for pinellia tuber excised tuber

Abstract

The invention discloses a highly effective revulsion induction method for pinellia tuber excised tuber comprising the steps of, using successive transfer culture base for transferred culture, using root-producing culture medium for root-producing culture, using in vitro tuber induction culture medium for in vitro tuber induced culturing, wherein the successive transfer culture base comprises MS minimum medium, cytokinin, 1-naphthalene acetic acid, agar powder and cane sugar, the root-producing culture base comprises MS minimum medium, 1-naphthalene acetic acid, activated charcoal, agar powder, cane sugar, the in vitro tuber induction culture medium comprises MS minimum medium, gibberellin, abscisic acid, propyl dihydro-jasmonate, cane sugar, Paclobutrazol, cytokinin, activated charcoal and agar powder.

Description

technical field

[0001] The invention relates to plant regeneration through tissue culture technology, in particular to a method for cultivating isolated pinellia tubers. Background technique

[0002] Pinellia.ternata.(Thumb)Breit is a perennial herbaceous plant of Araceae. The base forms a tuber, which is the main storage organ and reproductive organ. Containing various active ingredients such as alkaloids and pinellia protein, it has many pharmacological effects such as drying dampness and resolving phlegm, reducing adverse cough, anti-early pregnancy, anti-tumor, etc. It is a unique traditional Chinese medicinal material in my country and has a large demand. Due to over-collection, the wild resources of pinellia are almost exhausted. Pinellia has three reproductive methods: seed propagation, small tuber propagation and bulbil reproduction. In natural evolution, asexual reproduction has been formed. Therefore, small tubers are generally used as reproductive organs in artif...