Schizophrenia related nucleic acid sequence

A schizophrenia and nucleic acid sequence technology, applied in the field of a group of schizophrenia-related nucleic acid sequences, can solve problems such as no reports related to schizophrenia, and achieve the effect of improving diagnosis

Inactive Publication Date: 2006-02-22
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Through the retrieval of prior art documents, it is found that this field has just started in the world at present. In the Science Express magazine published on March 10 this year (Robert J.Klein, Caroline Zeiss, Emily Y.Chew, Jen-Yue Tsai, Richard S. Sackler, Chad Haynes, Alice K. Henning, John Paul SanGiovanni, Shrikant M. Mane, Susan T. Mayne, Michael B. Bracken, Frederick L. Ferris, Jurg Ott, Colin Barnstable, Josephine Hoh. Complement Factor H Polymorphism in Age-Related Macular Degeneration.Science Express Research Articles, 2005 March 10) (the role of compound factor H in age-related macular degeneration. Science Express. March 10, 2005), scientists led by Robert J. Klein through the study It was found that there is an obvious correlation between compound factor H and age-related macular degeneration, but no corresponding genome-wide association analysis has been carried out in the field of schizophrenia. So far, no single nucleotide in the above-mentioned group of 12 nucleic acid sequences Correlation report between polymorphic loci and schizophrenia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Collection and extraction of genes

[0055] The patients with schizophrenia were from the Shanghai Mental Health Center in China and the University of Aberdeen in the United Kingdom. The diagnostic criteria were DSM-IV (the second revision of the Chinese psychiatric classification scheme and diagnostic criteria). Matched control individuals were provided by the Outpatient Department of Shanghai Regional Hospital and the University of Aberdeen, UK. There were 172 patients with sporadic schizophrenia in China, the average age was 50.17±14.33 years old (standard deviation), and the onset age was 28.48±9.35 years old (standard deviation). There were 171 healthy volunteers in the control group, with an average age of 28.10±8.14 years (standard deviation). There were 166 patients with sporadic schizophrenia in the UK, the average age was 44.55±13.80 years old (standard deviation), and the age of onset was 24.33±8.25 years old (standard deviation). There were 179 ...

Embodiment 2

[0056] Example 2: Obtaining of Nucleotide Sequence and Detection of 12 Single Nucleotide Polymorphism Sites

[0057] All SNPs are from Affymetrix GeneChip Mapping 100KSet, the median physical distance is 8.5kb, and the average distance is 23.6kb.

[0058] 250ng of genomic DNA was digested with XbaI or HindIII at 37°C for 2 hours, inactivated at 70°C for 20 minutes, and the genomic DNA was converted into 200-2000bp nucleic acid fragments.

[0059] The digested DNA was ligated with 0.25 μM XbaI or HindIII adapter (Affymetrix) and 250 U T4 DNA ligase (New England Biolabs) at 16°C for 2 hours, inactivated at 70°C for 20 minutes, and ligated at the end of the restriction site for general amplification Primer adapter. .

[0060] The ligation product is divided into 3 wells, and the total volume of the reaction system in each well is 100ul, of which

[0061] MgCl 2 : 1.0mM

[0062] dNTP(each): 0.3mM

[0063] Platinum Pfx DNA Polymerase (Invitrogen Corporation): 0.05U / μL

[006...

Embodiment 3

[0067] Example 3: Correlation between 12 single nucleotide polymorphism sites and schizophrenia

[0068] Statistics: Using Varia1.0 (Silicon Genetics) software, 115,071 SNPs in the whole genome were divided into Blocks, and Haplotype was constructed for genome-wide association analysis. The statistical significance level was set at False Discovery Rate (FDR) < 0.01.

[0069] Results: Through the detection of single nucleotide polymorphisms in the Chinese population and the Scottish population, a total of 98,899 single nucleotide polymorphism sites were found in the two populations. There are 8,322 nucleotide polymorphic sites. The polymorphism detection results of 115,071 single nucleotides in the whole genome are shown in Table 1.

[0070] The proportions of the two populations based on 98899 common SNPs and 8322 population-specific SNPs

Crowd type

MAF * >0.01

MAF * >0.05

MAF * >0.1

SNPs

％

SNPs

％

SNP...

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PUM

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Abstract

The invention relates to a Schizophrenia related nucleic acid sequence, which comprises sequences represented in SEQ ID No.1-12 in the sequence table, wherein the existence of mononucleotide polymorphism at No.26 is G, and is A equivalent site on the DNA compatible chains, and if the existence of mononucleotide polymorphism at No.26 is C, and is T equivalent site on the DNA compatible chains.

Description

technical field [0001] The present invention relates to a group of nucleic acid sequences in the gene field of biotechnology, in particular to a group of nucleic acid sequences related to schizophrenia. Background technique [0002] Schizophrenia is a complex and destructive brain disorder with an incidence rate of about 1%, which seriously affects human health and consumes a lot of manpower, material and financial resources. Although the exact etiology of schizophrenia has not yet been found, sib pair analysis, family analysis, and parasite studies have shown that genetic factors play an important role in the pathogenesis. Genomic genetic linkage screening identified positive LOD values ​​at many loci on the chromosome, which ruled out a single major locus for schizophrenia. SNPs (single nucleotide polymorphisms) are the sequence changes of a single nucleotide at a specific position in the genome in a certain species. It is generally believed that there is one SNP per 1 K...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/68
Inventor 贺林秦炜师咏勇卞莉冯国鄞
Owner SHANGHAI JIAO TONG UNIV
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