Proteoglycan isolated from cartilaginous fish and process for producing the same

A technology of proteoglycan and cartilage, which can be applied to medical preparations containing active ingredients, peptide/protein components, bone diseases, etc. It can solve the problems that the tumor inhibition effect of shark cartilage is not clear, etc.

Inactive Publication Date: 2006-04-19
HOSOKAWA MICRON CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of the tumor suppressive effect of shark cartilage in the body through oral ingestion of shark cartilage is hardly clear, and it has not been possible to conclude that shark cartilage inhibits MMP in tissues through oral ingestion of shark cartilage.

Method used

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  • Proteoglycan isolated from cartilaginous fish and process for producing the same
  • Proteoglycan isolated from cartilaginous fish and process for producing the same
  • Proteoglycan isolated from cartilaginous fish and process for producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Isolation of Proteoglycans from Shark Cartilage

[0099] (1) Modulation of Shark Cartilage Extract

[0100] Sun-dried shark cartilage (cartilage of blue shark) was roughly pulverized with a coarse pulverizer (manufactured by Hosokawa Micron Co., Ltd., FM-1) to an average particle size of about 5 mm. The obtained coarsely pulverized product was pulverized together with liquid nitrogen using a freeze pulverizer (manufactured by Hosokawa Micron Co., Ltd., LX-1) to obtain a fine powder with an average particle diameter of 25 μm. The average particle diameter is measured using Microtrac 9320HRA (manufactured by Leedsand Northrup). 15 kg of distilled water was added to 3 kg of the obtained fine powder, stirred at room temperature for 20 minutes, and then centrifuged (5 minutes, 3000 rpm) to obtain a supernatant. 10 kg of distilled water was further added to the remaining residue, and after stirring at room temperature for 20 minutes, centrifugation was performed in the same...

Embodiment 2

[0132] Effects of intake of shark cartilage extract relative to cancer-bearing hamsters

[0133] (1) Preparation of experimental diet

[0134] As a basic food, CE-2 powder purchased from Japan CLEA Co., Ltd. was used. As an experimental food, the shark cartilage prepared in Example 1 (1) was added in 0.2% by weight or 0.4% by weight in the basic food. Extract (hereinafter, % by weight may be abbreviated as %). The following hamsters were free to eat the above-mentioned basal diet and experimental diet. Water was also available ad libitum. The body weight and bait intake of each hamster that ingested the experimental diet were measured. There were no significant differences in body weight and differences in bait intake among the same groups.

[0135] (2) Experimental animals

[0136] Syrian golden hamsters (6 weeks old) were purchased from Japan SLC Co., Ltd. Every 4-5 hamsters were put into a plastic cage for feeding in a rearing room adjusted to a room temperature of 24...

Embodiment 3

[0154] Amount of haptoglobin in hamster serum

[0155] The serum of non-cancer-bearing hamsters ingesting shark cartilage extract for 2 weeks was separated by SDS-PAGE, and CBB staining was performed. As a result, the band at about 80 kDa increased in the experimental diet group compared with the basal diet group. The above-mentioned 80kDa band was excised and subjected to structural analysis (protein sequencing: PPSQ21, manufactured by Shimadzu Corporation), and it was found to be hamster endogenous haptoglobin (α chain: VDLSNDAMDTADDS (sequence number: 1), β chain: IIGGSLDAKGSFPW (sequence number No.: 2)).

[0156] Haptoglobin has functions such as binding to oxidized hemoglobin produced by hemolysis, and neutralizing the toxicity of oxidative vascular disorders. Represented by hemolysis, it has recently become an indicator protein for many diseases such as the malignancy of cancer (Pathol Oncol Res.1998; 4(4) : 271-6, Br J Cancer. 1991 Aug; 64(2): 386-90). In addition, t...

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Abstract

It is intended to provide a cartilage extract which exerts its effect even in the case of taking in a small amount and shows a definite function mechanism of the effect. Namely, isolated proteoglycan which originates in a water extract of the cartilage of a cartilaginous fish and the main component of which has a molecular weight of 500 kDa or higher; a composition containing this proteoglycan; a medicinal composition containing the proteoglycan as the active ingredient; and a process for producing the proteoglycan which comprises the step of grinding the cartilage of a cartilaginous fish into pieces of an average diameter of 100 mum or less, the step of adding water to the ground matter and extracting water-soluble components therefrom, the step of separating the aqueous phase containing the water-soluble components, and the step of adding an alcohol to the aqueous phase and obtaining the precipitate.

Description

technical field [0001] The invention relates to a proteoglycan isolated from cartilaginous fishes and a production method thereof. Specifically, it relates to a proteoglycan useful in the medical field for maintaining health and improving the quality of life, and a method for producing the same. Background technique [0002] Cancer is a common disease that afflicts human beings, and its treatment is becoming a worldwide issue. In Japan, the main cause of death is shifting from infectious diseases to lifestyle-related diseases. In particular, cancer has become the number one cause of death in Japan, and research is ongoing as the most important issue in disease countermeasures. Cancer can occur in all organs and tissues, so it varies with constitution, gender, age, region and living habits. In addition, all properties including metastasis and proliferative ability differ according to the type of cancer, so no definitive treatment method has been established so far. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K38/55A61P17/06A61P19/02A61P27/02A61P27/06A61P29/00A61P35/00A61P43/00C07K14/46C12P21/00A61K35/60C08B37/00
CPCA61K35/60C07K14/461C08B37/0066A61P17/06A61P19/02A61P27/02A61P27/06A61P29/00A61P35/00A61P35/04A61P43/00
Inventor 佐藤健司堤雅弘野村义宏村田奈保近藤直幸
Owner HOSOKAWA MICRON CORP
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