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Coastal glehnia root extract with function of antagonizing gastric ulcer and its prepn

A kind of extract, the technology of Adenophora japonicus, applied in the field of medicine, achieves the effect of clear mechanism of action and simple preparation process

Inactive Publication Date: 2007-11-07
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the application of Adenophora or its extracts in the treatment of gastric ulcer or chronic gastritis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 5 kg of North Radix Ginseng coarse powder, add 35 L of water to soak, heat and reflux and extract 3 times, the extraction time is 2, 2, and 1 hour respectively, combine the water extract, filter, and concentrate under reduced pressure at 60 ° C to a relative density of 1.20 (50 ℃), to obtain a concentrated solution; add ethanol to the concentrated solution until the alcohol content is 90%, place, filter, merge the filtrate, reclaim the solvent under reduced pressure until the concentration of the crude drug contained in the solution is 50g / ml, filter; Adsorbed by macroporous adsorption resin, the amount of resin used is 6kg, the impurities are washed away with water and 20% ethanol respectively, and then eluted with 50% ethanol, the alcohol eluate is collected, the solvent is recovered under reduced pressure, vacuum dried and pulverized to obtain 81g of the extract .

Embodiment 2

[0017] Take 5 kg of North Radix Ginseng coarse powder, add 35 L of water to soak, heat and reflux extraction for 3 times, the extraction time is 2, 2, and 1 hour respectively, combine the extracts, filter, and concentrate under reduced pressure at 60 ° C to a relative density of 1.10 (50 ° C ), to obtain the concentrated solution; adding ethanol to the concentrated solution until the alcohol content is 60%, placing, filtering, merging the filtrate, reclaiming the solvent under reduced pressure until the concentration of the crude drug contained in the solution is 25g / ml, filtering; the filtrate is passed through Macroporous adsorption resin adsorption, resin consumption 10kg, wash away impurities with water and 20% ethanol respectively, then elute with 75% ethanol, collect ethanol eluate, recover solvent under reduced pressure, vacuum dry and pulverize to obtain 153g of the extract.

Embodiment 3

[0018] Example 3 Inhibitory Effect of Radix Ginseng Extract on Gastric Ulcer in Mice Induced by HCl / EtOH

[0019] (1) Experimental materials

[0020] Animals: 28 male KM mice (25±2g), fasted for 24 hours before administration, and allowed to drink water freely.

[0021] Reagents: 0.3M HCl / 60% EtOH, 0.2% CMCNa, 5% formalin, normal saline, cimetidine.

[0022] Materials and instruments: 1ml mouse gavage device, 1ml syringe, surgical scissors, tweezers, millimeter ruler, volumetric flask.

[0023] (2) Experimental method

[0024] Mice are randomly divided into 4 groups, 10 in every group: negative control group (0.2% CMCNa, 10ml / kg, i.g.), positive control group (cimetidine, 100mg / kg -1 , i.g.), drug group (200, 400mg / kg, i.g.). 50 minutes after the administration, 0.2ml of 0.3M HCl / 60%EtOH was given by intragastric administration to induce ulceration. One hour later, the stomach was taken out, 1ml of normal saline was injected to make it swell, and after 30 minutes of fixati...

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PUM

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Abstract

The present invention belongs to the field of medicine technology, and is especially Chinese medicinal material coastal glehnia root extract and its preparation process. The preparation process includes the main steps of water extraction, alcohol precipitation, macroporous resin adsorption, water washing and alcohol solution eluting. The extract contains three kinds of active component, including lignan glycoside, coumarin glycoside and cinepazid glycoside, and includes 21 kinds of compound, including glehlinoside A, bergaptol 5-O-beta-D-gentiobioside, etc. It has obvious effects of antagonizing gastro ulcer, resisting oxidation and relieving pain, and is used as material of medicine for treating gastric ulcer and chronic gastritis.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to extracts of the traditional Chinese medicine Adenophora japonicus and a preparation method thereof, and also relate to the medical use of the extracts of Adenophora japonicus for resisting gastric ulcer. Background technique [0002] Gastric ulcer is a common and frequently-occurring disease of the digestive system, and its incidence is on the rise in recent years. Its pathophysiological process is relatively complicated, generally speaking, it is caused by the imbalance between the destructive effect of attack factors on gastric mucosa and the protective effect of defense factors on gastric mucosa. Attacking factors include gastric acid, pepsin, refluxed upper small intestinal fluid, Helicobacter pylori, etc.; protective factors include gastric mucosal barrier and gastric mucus barrier. Gastric mucosal barrier not only refers to the protective effect of gastric mucosal epitheliu...

Claims

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Application Information

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IPC IPC(8): A61K36/23A61P1/04C07H1/08A61K125/00
Inventor 原忠李铣赵亚孔维雪王丽莉
Owner SHENYANG PHARMA UNIVERSITY
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