Method of purifying aqueous amide compound solution and process for producing amide compound

A technology of amide compounds and refining methods, applied in chemical instruments and methods, carboxylic acid amide separation/purification, organic chemistry, etc., can solve the problems of insufficient separation of foaming impurities and no reports, etc., to achieve less impurities and foaming small effect

Inactive Publication Date: 2006-04-26
MITSUBISHI CHEM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is called the copper catalyst method, and impurities (acrylamide oligomers, cross-linking substances) in the generated acrylamide aqueous solution are removed under reaction conditions that are likely to form impurities of acrylamide. Even the aqueous acrylamide solution obtained by using a biocatalyst cannot sufficiently separate foaming impurities
[0014] Therefore, for the aqueous solution of an amide compound obtained from a biocatalyst, an example of purification by an ultrafiltration membrane has not been reported so far.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, comparative example 1

[0043] The modulation of biocatalyst: Rhodococcus rhodochrousJ1 (FERM BP-1478) with nitrile hydratase activity is contained in containing 2% glucose, 1% urea, 0.5% peptone (peptone), 0.3% yeast extract, 0.05% cobalt chloride (both aerobic culture at 30° C. in a medium (pH 7.0). This was washed with a 50 mM (mol) phosphate buffer (pH 7.0) to obtain a suspension of bacterial cells (containing 15% by mass of dried bacterial cells).

[0044] The reaction of converting 3-cyanopyridine to nicotinamide under the action of bacteria: add 1L of 15 mass% 3-cyanopyridine aqueous solution (50mM phosphate buffer, pH7) into a 5L glass beaker, and add the adjusted 25 mL of bacterial cell suspension was slowly stirred in a water tank at 30°C. After 24 hours, a 17% aqueous solution of nicotinamide was obtained. Filtration was performed using a membrane filter with a pore size of 0.45 μm (manufactured by Advantec Co., Ltd., cellulose mixed ester ty...

Embodiment 2、3、4

[0048] Embodiment 2, 3, 4, comparative example 2, 3

[0049] Preparation of biocatalyst: Rhodococcus rhodochrous J1 (FERM BP-1478) cell suspension (containing 15% by mass of dry cells) having nitrile hydratase activity was prepared in the same manner as in Example 1.

[0050] Separately, a monomer aqueous solution of 30% by mass of acrylamide, 1% by mass of methylenebisacrylamide, and 4% by mass of 2-dimethylaminopropylmethacrylamide was prepared.

[0051] Next, the bacterial cell suspension, monomer aqueous solution, 10% by mass N,N,N',N'-tetramethylethylenediamine aqueous solution and 10% by weight ammonium persulfate aqueous solution were prepared at 5L / hr, 2L / hr, 0.1L / hr, and 0.1L / hr are linearly mixed in sequence, and the effluents are respectively filled into containers of 300×300×30mm. In the container, the monomers are polymerized to form a gel sheet (gel sheet) ).

[0052] The obtained cell-solidified gel block was finely divided into squares of about 0.5 mm with a...

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PUM

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Abstract

The invention relates to a method for refining an amide compound aqueous solution. The method is to filter the aqueous solution containing the amide compound prepared by a biocatalyst with an ultrafiltration membrane with a molecular weight cut-off greater than or equal to 1000 but less than 10000.

Description

technical field [0001] The present invention relates to a method for refining an aqueous solution of an amide compound prepared using a biocatalyst and a method for producing an amide compound using a biocatalyst. [0002] This application claims priority from Japanese Patent Application No. 2003-98139 filed on April 1, 2003, the contents of which are incorporated herein. Background technique [0003] The production method of a compound using a biocatalyst with enzyme activity has the advantages of simplifying the reaction process in order to stabilize the reaction conditions, and having few by-products so that the purity of the reaction product is high or the purification process can be simplified. used in the manufacture of. [0004] Since the discovery of nitrile hydratase, an enzyme that converts nitrile compounds into amide compounds, the production of compounds using biocatalysts has also been extensively studied in the production of amide compounds. The production o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02B01D61/14C07C231/24C07C233/09C07D213/82C12P17/06C12P17/12
CPCB01D61/145C07C231/24C07D213/82C12P13/02C12P17/12C07C233/09
Inventor 村尾耕三加纳诚
Owner MITSUBISHI CHEM CORP
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