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DNA chip for traditional Chinese medicine anti tumour mechanism rapid analysis and its method

A DNA chip and mechanism of action technology, applied in the field of bioengineering, can solve problems such as the inability to analyze large gene expression changes

Inactive Publication Date: 2006-05-03
哈尔滨基太生物芯片开发有限责任公司
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  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the defect that traditional molecular biology techniques cannot analyze a large number of gene expression changes at the same time, the present invention provides a DNA chip and its method for rapidly analyzing the mechanism of anti-tumor traditional Chinese medicine, which can shorten the drug research and development cycle and Reduce the cost of drug research and development, and accelerate the entry of traditional Chinese medicine into the international market

Method used

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  • DNA chip for traditional Chinese medicine anti tumour mechanism rapid analysis and its method
  • DNA chip for traditional Chinese medicine anti tumour mechanism rapid analysis and its method
  • DNA chip for traditional Chinese medicine anti tumour mechanism rapid analysis and its method

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specific Embodiment approach 1

[0005] Specific Embodiment 1: The gene types and parameters of the DNA chip for rapid analysis of the mechanism of action of anti-tumor traditional Chinese medicines in this embodiment are shown in Tables 1-2.

specific Embodiment approach 2

[0006] Specific embodiment two. This embodiment quickly analyzes the mechanism of action of anti-tumor traditional Chinese medicine according to the following steps: 1. Select anti-tumor traditional Chinese medicine as a tool drug, they can be arsenous acid injection, paclitaxel injection, parasitic polypeptide, hibiscus acid , 5,7,4-trihydroxyisoflavone, soybean saponin, soybean sterol, etc., or one or more of them. Two, the cell lines used in the present invention to screen anti-tumor drugs are tumor cell lines commonly used at present, and they are promyelocytic leukemia HL-60, chronic myelogenous leukemia K-562, liver cancer HepG2, gastric adenocarcinoma BGC823, breast cancer Bcap -37. One or more of ovarian cancer HO8910, prostate cancer PC3, cervical cancer Hela, etc. 3. After using the above-mentioned anti-tumor traditional Chinese medicines to act on the cell models, use the CCK8 method to screen out the sensitive cell lines corresponding to each tool drug, based on it...

specific Embodiment approach 3

[0009] Specific embodiment three: In this embodiment, 5,7,4-trihydroxyisoflavone is used as an example to describe the present invention in detail:

[0010] 1. Preparation of DNA chip: 1. In this embodiment, 17,000 PCR amplification products are used as spotting DNA (see Table 1-2 for gene type and parameters), and the PCR product is dissolved with 50% DMSO (dimethyl sulfoxide). To a concentration of 0.2 μg / μl to 0.5 μg / μl, it becomes a working solution for spotting (Note: PCR products should be strained before spotting: 99.9°C for 5 minutes, and quickly placed on ice). 2. Arrange the spotting working solution according to the required dot matrix pattern, transfer it to a 384-well microplate, and start to spot the sample (temperature 20-23°C, relative humidity 40-50%). Aldehydated slides were spotted. 3. After spotting the samples, place the slides at room temperature under dust-proof conditions overnight, preferably for a week. 4. Take out the slides that have been placed o...

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Abstract

The DNA chip with opposite method to express analyze the action mechanism of anti-tumor Chinese traditional medicine overcomes the shortcoming that it can not analyze a mass of gene expression variety. Wherein, the chip relates to apoptosis, periodic control, and drug transformation metabolism. The analysis method comprises: with objective drug as tool drug and tumor cell strain as cell model, using said Chinese drug to act on the model; screening the sensitive cell line opposite to tool drug; detecting drug-acted gene with gene expression spectrum chip; stating and classifying gene with bioinformation science; according to drug label gene and change low of special tumor gene, validating the mechanism on molecular cell level.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a DNA chip capable of rapidly analyzing the action mechanism of anti-tumor traditional Chinese medicines and a technology thereof. Background technique [0002] Malignant tumor is a major disease that endangers human health, and has become the second leading cause of death in various diseases after cardiovascular disease. In my country, about 900,000 cancer patients die every year, and the number of deaths has been increasing year by year in recent years. Therefore, countries all over the world have invested heavily in research and development of anti-tumor drugs. It is obvious that the market prospect of antineoplastic drugs is promising. Although dozens of chemotherapeutic or adjuvant antineoplastic drugs can be used in clinical treatment so far, most of these drugs can only alleviate the disease, but cannot achieve the purpose of cure. Especially most com...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 韩俊李昌利顾雪锋李克深闫作梅于小丽杨光
Owner 哈尔滨基太生物芯片开发有限责任公司
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