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Cultivation of peony germinal dislocation

An in vitro culture and peony technology, applied in the field of plant tissue culture, can solve problems such as lack of application research, and achieve the effects of improving breeding efficiency, high seedling rate, and reducing input of human and material resources.

Inactive Publication Date: 2006-05-31
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The in vitro culture of immature embryos has not been reported so far, and there is even a lack of research on the application of this technology in breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Explants obtained

[0024] The purple peony carpels collected from healthy plants were taken back to the laboratory, rinsed with running water for 24 hours, thoroughly scrubbed with Xishuiling (White Cat brand), the ovules were peeled off, and disinfected on an ultra-clean bench. The ovules were first treated with 70% ethanol for 3 minutes, washed 3 times with sterilized distilled water, then treated with 0.2% NaClo for 20 minutes, washed 3 times with sterilized distilled water, peeled off the seed coat, broke open the endosperm, and picked out the seed embryo with a dissecting needle for inoculation .

Embodiment 2

[0026] (1) Cultivate for 30 days on the explant starting medium Q-1, then completely subculture (I) to J-2, cultivate for 42 days, true leaves are drawn out on J-2, and the root length is greater than the seedling of 1.5cm Progeny (II) to J "-1; the seedlings whose roots are shorter than 1.5cm on J-2 are subcultured (II) to J "-2;

[0027] (2) The seedlings on J"-1 were refrigerated at 4°C for 60 days, and hardened in the greenhouse; the rooted seedlings on J"-2 were refrigerated at 4°C for 90 days, and hardened in the greenhouse; the unrooted seedlings were discarded. Culture conditions: culture temperature 25±1℃, light 20h / d, light intensity 1600Lx.

Embodiment 3

[0029] (1) After the explants were cultured for 40 days on the starting medium Q-2, 3, and 4, they were equally divided into 4 parts and subcultured (1) to J-1, 3, 4, and 5 respectively;

[0030] (2) The seedlings on J-3, 4, and 5 were immediately placed in the dark at about 15 ± 1°C for root induction for 15 days;

[0031] (3) Then the seedlings are all subcultured (II) to J-1 again; after 30 days, all the subcultured (III) is then J"-1;

[0032] (4) After growing on J"-1 for 30 days, pick out the seedlings with good root and leaf growth for hardening. Culture conditions: culture temperature 25±1°C, light 20h / d, light intensity 2000Lx.

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PUM

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Abstract

An in-vitro culture method for peony embryo bud includes such steps as disinfecting the explant, culturing in starting culture medium for 30-40 days, culturing in secondary culture medium A for 35-45 days, culturing in the secondary culture media B and C, and growing in green house.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for in vitro culture of peony embryos. Background technique [0002] Peony is a traditional famous flower native to our country, and it is currently the only candidate for the "national flower". It not only occupies an important position in the domestic flower market, but also is an important export flower of our country. However, its reproduction and breeding are relatively difficult (Buchheim JAT et al, 1992). Breeding is still carried out in the traditional hybridization method, but the hypocotyls of peony seeds all have dormancy phenomenon, and it takes 1 to 2 years for the seeds to germinate in a natural state. , and the germination rate is low and the variation is large, it is difficult to obtain uniform seedlings (Zilis MR et al, 1976; Barton LV et al, 1957). Although the GA3 treatment can break the dormancy of the upper and lower hypocotyls of some peony seeds an...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 成仿云何贵梅
Owner BEIJING FORESTRY UNIVERSITY