Young schistosomiasis cell style vaccine for preventing from schistosomiasis disease
A technology for schistosomiasis and schistosomiasis, which is applied in the field of schistosomiasis cell-based vaccines, can solve the problems of impracticality, unsafe cercariae live vaccine consumption, poor resistance to attacking infected worms, etc., and achieves easy production and preparation , Overcome the large amount of materials consumed and the effect of overcoming insecurity
Inactive Publication Date: 2006-05-31
CENT SOUTH UNIV
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Because the epidemic and transmission of the disease among humans and animals is caused by the natural environment, the current prevention and control measures cannot meet the requirements of controlling its epidemic and transmission.
[0003] So far, domestic research on (Japanese/Mansoni) schistosomiasis vaccine types includes: 1. Live attenuated vaccine: live cercariae of schistosomiasis are used to vaccinate/immunize animals after being irradiated with radiation. 60% to 90% (worm reduction rate) protective immunity, but very impractical; 2. Insect vaccine: including immunizing animals with tissues or soluble compound components of Schistosoma adult or juvenile worms, which can hardly induce the host to produce resistance Attack infection immunity; 3. Natural molecular vaccines: isolate effective natural ingredients from schistosome worms or eggs, add adjuvants and use them to immunize animals, which can only induce partial protective immunity, and immature egg molecules are used The antigen has anti-schistosome reproduction or egg embryo development effect, but the anti-challenge infection is poor; 4. Recombinant vaccine: Antiserum is obtained after screening and identifying effective antigen molecules or irradiating cercariae to immunize animals, and screening the cDNA library of schistosomiasis , using genetic recombination technology to express recombinant/fusion proteins to immunize animals, the protective immune effect is within 40% (worm reduction rate); 5. Gene vaccine: also known as DNA vaccine, screened by library (including polypeptide library) The ident
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[0016] 1. Preparation of Pediatric Worm Cell Vaccine
[0017] The cercariae of Schistosoma japonicum were used to perfuse the healthy rabbits on the 14th to 24th day after the abdomen was heavily infected. After the worms were collected and washed, the worms were quickly chopped under aseptic conditions, and 10 times the worms containing 0.25% trypsin and 0.02% EDTA digestion solution was digested for 30min, the supernatant was aspirated, and Ca 2+ , Mg 2+ The D-Hank's solution was used to stop the digestion; the obtained cells were centrifuged at 2000rpm×5min, washed with D-Hank's repeatedly for 3 times, the non-cellular components in the supernatant were discarded, and then a small amount of PBS or D-Hank's solution was added for cell counting , and set the volume to 1.6×10 7 / ml, used as the immunization dose concentration. The living cells of Schistosoma japonicum hepatic stage juveniles were obtained, and the somatic cells of Schistosoma japonicum were obtained accordi...
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A schistosomulum cell type vaccine for preventing schistosomiasis is prepared through collecting the schistosomulums from the rabit infected by Japanese schistosome for 14-24 days, fast cutting in aseptic mode, digestion by pancreatin, taking supernatant, adding Ca or Mg ion type D-Hankí»s liquid to terminate the digestion to obtain living cells, freezing them to obtain the dead cells and using said living cells or dead cells as vaccine.
Description
technical field [0001] The invention relates to a vaccine product for immunization and prevention of schistosomiasis, in particular to a childhood worm cell vaccine. Background technique [0002] Schistosomiasis is a major tropical disease in the world, threatening 600 million people. Among them, schistosomiasis japonicum is seriously prevalent in our country, which is a zoonotic parasitic disease. Because the epidemic and transmission of the disease among humans and animals is caused by the natural environment, the current prevention and control measures cannot meet the requirements of controlling its epidemic and transmission. [0003] So far, domestic research on (Japanese / Mansoni) schistosomiasis vaccine types includes: 1. Live attenuated vaccine: live cercariae of schistosomiasis are used to vaccinate / immunize animals after being irradiated with radiation. 60% to 90% (worm reduction rate) protective immunity, but very impractical; 2. Insect vaccine: including immunizi...
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Login to view more IPC IPC(8): A61K39/002A61P33/12
CPCY02A50/30
Inventor 曾庆仁林雪迟蔡立汀龚燕飞张祖萍
Owner CENT SOUTH UNIV
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