Method for producing lactic acid bacteria formulation and lactic acid bacteria bacteriocin using ethanol draff liquid

A technology of lactic acid bacteria bacteriocin and waste liquid, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve problems such as waste of resources, and achieve the effects of increasing utilization value, extending industrial chain and reducing production costs.

Inactive Publication Date: 2006-07-26
大连春天生物技术有限公司
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] Aiming at the above problems, the present invention provides a method for producing lactic acid bacteria preparations and lactic acid bacteria bacteriocins by using alc

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing lactic acid bacteria formulation and lactic acid bacteria bacteriocin using ethanol draff liquid

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0050] Example 1 Production of Lactic Acid Bacteria Preparation by Submerged Fermentation of Corn Alcohol Waste Grains Liquid

[0051] 1. Preparation of the filtrate of corn alcohol waste grains

[0052] Collect fresh alcohol waste grains, centrifuge it in a horizontal centrifuge (WL450 produced by Sichuan Jiangbei Machinery Factory), separate solid and liquid, take the separated liquid and filter it through a plate and frame filter, and collect the filtrate, which is the alcohol waste grains filtrate.

[0053] 2. Process conditions of lactic acid bacteria fermentation

[0054] 1. Expansion process of lactic acid bacteria:

[0055] (1) Production strain:

[0056] Lactobacillus gasseri 1-7;

[0057] Lactobacillus crispatus 23-5;

[0058] Enterococcus faecalis Entercococcus faecalis 23-7;

[0059] Freeze-dried, preserved strains, provided by the Institute of Industrial Microbiology, Liaoning Normal University.

[0060] Proportion of liquid culture medium: add 0.5% yeast extract to t...

Example Embodiment

[0070] Example 2 Production of lactic acid bacteria by submerged fermentation of corn alcohol waste grains

[0071] 1. Preparation of the filtrate of corn alcohol waste grains

[0072] Collect fresh alcohol waste grains, centrifuge it in a horizontal centrifuge (WL450 produced by Sichuan Jiangbei Machinery Factory), separate solid and liquid, take the separated liquid and filter it through a plate and frame filter, and collect the filtrate, which is the alcohol waste grains filtrate.

[0073] 2. Process conditions of lactic acid bacteria fermentation

[0074] 1. Expansion process of lactic acid bacteria:

[0075] (1) Production strain:

[0076] Lactococcus lactis. LN992;

[0077] Lactobacillus brevis;

[0078] Freeze-drying and preservation of bacteria were provided by the Institute of Industrial Microbiology, Liaoning Normal University.

[0079] (2) Liquid culture medium:

[0080] Add 0.5% yeast extract to the filtrate of corn alcohol spent grains; add 0.5% sucrose; PH5.5-6.0 15 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

This invention discloses a method for preparing lactobacillus preparation and lactobacillus bacteriocino with waste alcohol distiller liquid, and it belongs to bio-preparation prosessing sphere, comprising steps of: get corn alcohol distiller liquid after alcoholic fermentation; solid-liquid separate the alcohol distiller liquid to get clear licquid; obtain expand-culture medium from the clear licquid; expand culture bacterial gradually; obtain fermention medium from the clear licquid; ferment the expand-culture bacterial in fermention medium; lastly separate fermention liquid to get lactobacillus preparation and lactobacillus bacteriocino. which has high added value and is produced by using alcohol distiller liquid, especially corn alcohol distiller liquid. It can prolongs industrial chain of corn processing; can improves corn's value in use; can recycle resource and can depress the manufacturing cost of lactobacillus preparation and lactobacillus bacteriocino.

Description

technical field [0001] The invention relates to a processing method of lactic acid bacteria preparations and lactic acid bacteria bacteriocins in the field of biological preparation processing, more specifically, relates to a method for producing lactic acid bacteria biological preparations and lactic acid bacteria bacteriocins by using alcohol waste liquid, especially corn alcohol waste liquid method. Background technique [0002] Alcohol waste liquid (Vinasse) is a by-product of alcohol production by fermentation and is a kind of high-concentration organic wastewater. my country's annual emissions are as high as 30 million tons or more, which is not only a great waste of resources, but also seriously pollutes the environment. [0003] Lactobacillus bacteriocin is an active substance produced by certain lactic acid bacteria (Lactococcus and Lactobacillus), that is, a type of polypeptide or precursor polypeptide with antibacterial activity produced through the ribosome synt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12R1/225
Inventor 曹文伟
Owner 大连春天生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap