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Method for producing lactic acid bacteria formulation and lactic acid bacteria bacteriocin using ethanol draff liquid

A technology of lactic acid bacteria bacteriocin and waste liquid, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve problems such as waste of resources, and achieve the effects of increasing utilization value, extending industrial chain and reducing production costs.

Inactive Publication Date: 2006-07-26
大连春天生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] Aiming at the above problems, the present invention provides a method for producing lactic acid bacteria preparations and lactic acid bacteria bacteriocins by using alcohol waste liquid as a by-product, which solves the problem that the waste liquid is usually discharged as waste after corn processing alcohol, resulting in waste of resources.

Method used

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  • Method for producing lactic acid bacteria formulation and lactic acid bacteria bacteriocin using ethanol draff liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Production of Lactic Acid Bacteria Preparations by Submerged Fermentation of Corn Alcohol Waste Grains

[0051] 1. Preparation of corn alcohol waste residue filtrate

[0052] Collect fresh alcohol waste liquid, centrifuge through a horizontal centrifuge (WL450 produced by Sichuan Jiangbei Machinery Factory), separate solid and liquid, take the separated liquid and filter it through a plate and frame filter, and collect the filtrate, which is the alcohol waste liquid filtrate.

[0053] Second, the process conditions of lactic acid bacteria fermentation

[0054] 1. Lactic acid bacteria strain expansion process:

[0055] (1) Production strain:

[0056] Lactobacillus gasseri 1-7;

[0057] Lactobacillus crispatus 23-5;

[0058] Enterococcus faecalis 23-7;

[0059] Freeze-dried and preserved strains were provided by the Institute of Industrial Microbiology, Liaoning Normal University.

[0060] The ratio of the liquid strain expansion culture medium: add 0.5% y...

Embodiment 2

[0070] Example 2 Production of Lactic Acid Bacteria Bacteriocins by Submerged Fermentation of Corn Alcohol Waste Grains

[0071] 1. Preparation of corn alcohol waste residue filtrate

[0072] Collect fresh alcohol waste liquid, centrifuge through a horizontal centrifuge (WL450 produced by Sichuan Jiangbei Machinery Factory), separate solid and liquid, take the separated liquid and filter it through a plate and frame filter, and collect the filtrate, which is the alcohol waste liquid filtrate.

[0073] Second, the process conditions of lactic acid bacteria fermentation

[0074] 1. Lactic acid bacteria strain expansion process:

[0075] (1) Production strain:

[0076] Lactococcus lactis (Lactococcus lactis.LN992);

[0077] Lactobacillus brevis;

[0078] Freeze-dried and preserved strains were provided by the Institute of Industrial Microbiology, Liaoning Normal University.

[0079] (2) Liquid strain expansion culture medium:

[0080] Add 0.5% yeast extract to the filtrate ...

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Abstract

This invention discloses a method for preparing lactobacillus preparation and lactobacillus bacteriocino with waste alcohol distiller liquid, and it belongs to bio-preparation prosessing sphere, comprising steps of: get corn alcohol distiller liquid after alcoholic fermentation; solid-liquid separate the alcohol distiller liquid to get clear licquid; obtain expand-culture medium from the clear licquid; expand culture bacterial gradually; obtain fermention medium from the clear licquid; ferment the expand-culture bacterial in fermention medium; lastly separate fermention liquid to get lactobacillus preparation and lactobacillus bacteriocino. which has high added value and is produced by using alcohol distiller liquid, especially corn alcohol distiller liquid. It can prolongs industrial chain of corn processing; can improves corn's value in use; can recycle resource and can depress the manufacturing cost of lactobacillus preparation and lactobacillus bacteriocino.

Description

technical field [0001] The invention relates to a processing method of lactic acid bacteria preparations and lactic acid bacteria bacteriocins in the field of biological preparation processing, more specifically, relates to a method for producing lactic acid bacteria biological preparations and lactic acid bacteria bacteriocins by using alcohol waste liquid, especially corn alcohol waste liquid method. Background technique [0002] Alcohol waste liquid (Vinasse) is a by-product of alcohol production by fermentation and is a kind of high-concentration organic wastewater. my country's annual emissions are as high as 30 million tons or more, which is not only a great waste of resources, but also seriously pollutes the environment. [0003] Lactobacillus bacteriocin is an active substance produced by certain lactic acid bacteria (Lactococcus and Lactobacillus), that is, a type of polypeptide or precursor polypeptide with antibacterial activity produced through the ribosome synt...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
Inventor 曹文伟
Owner 大连春天生物技术有限公司
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