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Biomolecule interaction analyzing method based on solid phase surface intensified light scattering

A technology of biomolecules and solid-phase surfaces, which is applied to the measurement of scattering characteristics and the preparation of test samples. It can solve the problems of poor anti-interference ability, impossibility, and many interference components, so as to expand the scope of application, low analysis cost, and Analyze the effect of high sensitivity

Inactive Publication Date: 2006-08-02
SOUTHWEST UNIVERSITY
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Problems solved by technology

However, although the scattering analysis technology based on the solution itself has high sensitivity, simple instruments and easy operation, it has fatal flaws.
1. Poor selectivity and poor anti-interference ability
In the bulk solution, all substances that can scatter light will bring great interference to the analysis of the analyte
2. The currently used scattering techniques are all measured in aqueous solution, but the analysis that needs to be carried out in oil-soluble reagents cannot be carried out, so the scope of analysis is limited to a certain extent
3. Due to the many interfering components in the bulk solution, it is also difficult to analyze the interaction of biomolecules

Method used

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  • Biomolecule interaction analyzing method based on solid phase surface intensified light scattering

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Embodiment Construction

[0018] The implementation process of the present invention will be described in detail below by using the analysis method of the present invention to analyze antigens and antibodies as an example:

[0019] (1) Use ordinary concave glass slides, the size of which is 25mm×70mm, and the diameter of the concave surface is 10mm;

[0020] (2) After the slide is fully washed with concentrated sodium hydroxide, concentrated sulfuric acid and distilled water, the concave surface of the slide is silanized with an aminopropyltrimethoxysilane reagent;

[0021] (3) Use 5% glutaraldehyde as a coupling agent to fix the biomolecular probe---goat anti-human IgG (antibody) on the concave surface of the glass slide;

[0022] (4) Drop biological target molecule----human IgG (antigen) solution on the concave surface of the glass slide, and adsorb the target molecule on the glass through the specific reaction between the probe and the target molecule (that is, the specific reaction of antigen and a...

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Abstract

The present invention relates to an analysis method capable of utilizing interaction produced by bioprobe molecule and biotarget molecule on the slide surface to intensify light-scattering signal to implement qualitative and quantitative determinations and intermolecular interaction character analysis. Said method includes the following processes: pretreatment of concave slide, fixation of probe molecule, combination of bioprobe and biotarget molecule, adopting synchronous scanning mode on the general spectrofluorometer to obtain scattering spectrum, utilizing said scattering spectrum to make qualitative and quantitative determinations of biotarget molecule and analysis of intermolecular intraction character and regeneration of bioprobe.

Description

technical field [0001] The invention belongs to optical analysis technology, and specifically relates to an analysis method for the qualitative and quantitative determination of biomolecules and the analysis of the interaction characteristics of biomolecules, which can be widely used in proteins, High sensitivity and high selectivity quantitative determination of nucleic acids, enzymes, cells and bacteria, biochemical drugs, etc., and protein and protein (such as antigen and antibody, donor and acceptor, protein and enzyme, etc.) DNA and DNA, DNA and protein ( Including specific interaction analysis between enzymes). Background technique [0002] The phenomenon of light scattering widely exists in the interaction process between light and particles. Existing research results have shown that the recognition, assembly and aggregation of biomacromolecules lead to a strong resonance light scattering signal (RLS) enhancement, and the signal enhancement is proportional to the con...

Claims

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Application Information

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IPC IPC(8): G01N21/51G01N1/28
Inventor 黄承志赵华文李原芳
Owner SOUTHWEST UNIVERSITY
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