Method for extracting small molecule collagen protein from fish scale utilizing enzyme engineering technique

A fish scale collagen and collagen technology, applied in the field of deep processing of fish scales, can solve the problems of amino acid loss and destruction, large molecular weight of fish scale collagen, complicated production process, etc., and achieve the effects of delaying aging, non-toxic effect and simple production process.

Inactive Publication Date: 2006-08-09
海南华研胶原科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Fish scale collagen has been widely studied in Japan, the United States, Norway and other countries since the 1990s. After entering the 21st century, domestic research on the extraction of collagen from fish scales has also increased. However, no matter at home or abroad, they produce fish scale collagen. A large amount of acid and alkali treatment is required, and acid and alkali treatment generally have the following problems: long production cycle, a large amount of acid and alkali treatment, serious pollution, complex production process, large equipment investment, large floor area, loss of amino acids and serious damage, the large molecular weight of the obtained fish scale collagen, which is not conducive to human body absorption and other shortcomings
At present, there is no report on the technology of using fish scales to produce small molecule collagen without acid and alkali treatment at home and abroad.

Method used

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  • Method for extracting small molecule collagen protein from fish scale utilizing enzyme engineering technique

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Experimental program
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Effect test

Embodiment 1

[0023] Accurately weigh 1 kg of washed and dried fish scales, send them into a pulverizer for crushing, and pass through a 100-mesh standard sieve, add 8 kg of distilled water to the sieved fish scale powder, and stir evenly until the fish scale powder becomes a suspension . Heat the suspension to 35°C-70°C, add 10 grams of neutral protease under natural pH state for enzymolysis, the enzymolysis time is 5 hours, after the enzymolysis is complete, raise the temperature to 90°C and keep for 10 minutes to inactivate the enzyme. Then, the enzymolysis solution is filtered to obtain supernatant and filter residue, and the supernatant is concentrated and dried to obtain small molecule fish scale collagen.

Embodiment 2

[0025] Accurately weigh 2 kg of washed and dried fish scales, send them into a pulverizer for crushing, and pass through a 120-mesh standard sieve, add 20 kg of distilled water to the sieved fish scale powder, and stir evenly until the fish scale powder becomes a suspension . Heat the suspension to 35°C-70°C, adjust the pH to 8.0 with 10% lye, add 10 grams of alkaline protease for enzymolysis, and the enzymolysis time is 8 hours. Minutes to inactivate the enzyme, then filter the enzymolysis solution to obtain supernatant and filter residue, the supernatant is concentrated and dried to obtain small molecule fish scale collagen.

Embodiment 3

[0027] Accurately weigh 5 kg of washed and dried fish scales, send them into a pulverizer for crushing, and pass through a 120-mesh standard sieve, add 50 kg of distilled water to the sieved fish scale powder, and stir evenly until the fish scale powder becomes a suspension . Heat the suspension to 35°C-70°C, adjust the pH to 7.5 with 10% lye, add 10 grams of alkaline protease and 10 grams of papain at the same time for enzymolysis, the enzymolysis time is 4 hours, and the enzymolysis is complete. The temperature is raised to 90° C. and kept for 10 minutes to inactivate the enzyme, and then the enzymolysis solution is filtered to obtain a supernatant and filter residue, and the supernatant is concentrated and dried to obtain small molecule fish scale collagen.

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Abstract

This invention relates to a method for extracting small molecular collagens from scales by an enzyme engineering technology including taking scales as the main raw material and extracting small molecular collagens from the scales by a biological enzyme cutting technology, which are prepared by selecting and cleaning scales, drying and crushing them to be sieved, mixed with water, enzymolyzed with proteinases then to eliminate enzymes and filter them to get the solution to be concentrated, dried to get the product.

Description

[0001] (1) Technical field: the present invention relates to a kind of deep processing technology of fish scale, especially a kind of technology that utilizes enzyme engineering technology to produce small molecule collagen protein with fish scale as raw material. (two) background technology: [0002] Fish scales are the deformation of the dermis of fish. The medicinal value of fish scales is mentioned in ancient medical books such as "Kaibao Materia Medica", "Meeting Medical Mirror", "Yilin Urge", "Compendium of Materia Medica", "Compendium Supplements" and other books. Also discussed the medical value of fish scales, pointed out: scales are sweet, salty and flat in nature and taste. Functions and Indications: Nourish blood, disperse blood, stop bleeding; treat hematemesis, aplastic anemia, metrorrhagia, stasis abdominal pain, hemorrhoids; treat sores, unknown swelling, mastitis, burns, tonify kidney and strengthen essence; treat hemophilia , leukemia, postpartum haemorrhage,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02
Inventor 程云开凌海军
Owner 海南华研胶原科技股份有限公司
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