Method for transferring bone marrow filling dry cell by TPO/FL

A bone marrow mesenchymal and stem cell technology, applied to cells modified by introducing foreign genetic material, using vectors to introduce foreign genetic material, recombinant DNA technology, etc., to achieve the effect of improving self-renewal in vitro and reducing costs
CN1818071AInactive Publication Date: 2006-08-16ZHEJIANG UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2006-08-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provided a method of transducing the marrow stem cell by the TPO / FL gene. (1) The mRNA of the TPO and the FL is separated from the human fetus liver, then to synthesis the two cDNA by the RT-PCR and clone it to the pBluescript carrier to construct the FL-IRES-TPO fragment, then to transform the fragment to the reverse transcription virus carrier to construct the pfl30 expression carrier. (2) transducing the marrow stem cell by the pfl30 expression carrier to construct the marrow stem cell with the high expression of the exogenous TPO and the FL. The stem cell is in the hemopoietic stem / ancestor cell in vitro culture system, so it can save the cost and improve the refresh, amplified and differential ability of the hemopoietic stem cell.
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Description

technical field

[0001] The invention belongs to biological technology and relates to a new biological technology for constructing bone marrow mesenchymal stem cells exogenously expressing thrombopoietin (TPO) and fms-like tyrosine kinase-3 ligand (FL). This technique enables bone marrow mesenchymal stem cells to highly express two cytokines, TPO and FL, thereby improving the ability to support the expansion of hematopoietic stem / progenitor cells in vitro. Background technique

[0002] The existing hematopoietic stem / progenitor cell in vitro expansion technology methods can be roughly classified into two categories: the first method is to add different combinations of recombinant cell growth factors in the culture system, the purpose is to use cell growth factors to stimulate hematopoietic stem / progenitor cells. proper regulation of proliferation and differentiation. Such technical methods can increase the expansion factor of hematopoietic stem / group cells to a certain exten...

Claims

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