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Immunity chromatography test paper for detecting farcina Boeck Hold's bacteria infection and prepn. method thereof

The technology of immunochromatographic test paper and Holderella is applied in the field of immunochromatographic test paper for detecting Burkholderia pseudomallei, and can solve the problem that no immunochromatographic test paper has been used to detect Burkholderia pseudomallei. Deerella and other problems, to achieve the effect of simple sample processing

Inactive Publication Date: 2006-09-20
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] There is no report on the detection of Burkholderia mallei with immunochromatographic test strips

Method used

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  • Immunity chromatography test paper for detecting farcina Boeck Hold's bacteria infection and prepn. method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the preparation of the immunochromatographic test paper that detects Burkholderia mallei

[0034] 1. Preparation of Burkholderia mallei specific antibody

[0035] 1) Preparation of Burkholderia mallei antigen

[0036] Inoculated on a common agar plate containing 4% glycerol (preservation number 350018), cultured in a 37°C incubator, observed the colony shape on the plate, picked a typical single colony, and transferred it to a slant of 4% glycerol common agar, and incubated it in a 37°C incubator Incubate for 24-28 hours. Wash the viscous bacterial lawn with 5% formaldehyde saline, put it in a saline bottle, and place it at 37°C overnight. The sterility test was observed for 7-8 days. After it was proved that there were no live bacteria, the bacterial solution was centrifuged at 10,000g-12,000g for 10-15 minutes, and the precipitate was collected, and the turbidimetric concentration (about 10 9 cfu bacteria / ml), that is, Burkholderia mallei antigen.

...

Embodiment 2

[0054] Embodiment 2, the detection of melioid bacteria and the cross test with other relevant bacteria

[0055] 1. Detection of melioidosis

[0056] 1) Provided by the Microbiological Testing and Research Center of the Institute of Microbial Epidemiology, Academy of Military Medical Sciences, 350018 strains of melioides and 350019 strains of melioides, both at a concentration of 1×10 6 cfu / ml, the bacterial suspension is used as the sample detection solution for later use.

[0057] 2) The results of the immunochromatographic test papers prepared in Example 1 for the specific antibody of the coated mallet bacterium and the goat anti-rabbit IgG were all positive.

[0058] 2. Cross-test with other related bacteria

[0059] 1) Provided by the Microbiological Testing and Research Center of the Institute of Microbial Epidemiology, Academy of Military Medical Sciences, 350,102 strains of melioidosis and 430,023 strains of Pseudomonas aeruginosa, both at a concentration of 1×10 8 c...

Embodiment 3

[0061] Embodiment 3, laboratory assessment

[0062] 1. Sample preparation

[0063] Different bacteria were inoculated in their appropriate culture medium and cultured under different conditions. After the bacterial lawn grew, it was washed with sterile saline, and the turbidimetric method was used to prepare a bacterial suspension of 1 × 10 6 cfu / ml, 1×10 7 cfu / ml and 1×10 8 cfu / ml, other bacteria suspension 1×10 8 cfu / ml, as a test solution for later use.

[0064] 2. Experimental method

[0065] Take the rapid detection reagent for the melioid bacteria antigen, add 3 drops (about 150 μl) of the above-mentioned sample detection solution to the sample pad, and start to observe the results after 2 minutes, and stop the observation after 15 minutes. Result report: if one precipitation line appears at the quality control line, it is negative, that is, there is no malleosis antigen detected; if two precipitation lines appear at the test line and the quality control line, it is...

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Abstract

This invention discloses immune chromatography test paper and its preparation method that used to detect glanders bokehuoerde germ. The paper includes sample mat 1, golden mark mat that nearly connected to one end of the mat 1 and contains glanders bokehuoerde germ antibody mark colloidal gold probe 2, nitrate cellulose film 3 that connected to another end of the gold mat and water absorbing mat 4 connected to another end of the NC film, the NC film is coated by separated detection line 5 and quality control line 6, the detecting line is glanders bokehuoerde germ antibody, the quality control line is rabbit antibody. This invention is used to the antibody detecting of clinical specimen, pollution and environmental glanders bokehuoerde germ, also can be used to identification of pure culture glanders bokehuoerde germ.

Description

technical field [0001] The invention relates to a test paper for detecting Burkholderia mallei, in particular to an immunochromatographic test paper for detecting Burkholderia mallei and a preparation method thereof. Background technique [0002] Melioidosis is a deadly and highly contagious bacterial disease caused by the bacterium Burkholderia mallie. Malleus was once named as Pfeifferella mallei (Pfeifferella mallei, 1918), Malleonydes mallei (Malleonydes mallei, 1933), Actinomyces mallei (Adtinoei mallei, 1933), Loefferella mallei (Loefferella mallei, 1935), Acinetobacter mallei (Acinetobacter mallei, 1964) and Pseudomonas mallei (Pseudomonas, mallei1966), named Burkholderia mallei in 1993. [0003] Because the gangrene bacteria are easy to obtain, they are highly pathogenic and have a high fatality rate. If not treated in time, the fatality rate can reach as high as 100%. Recognized as a classic biological warfare agent, it has been included in the checklist of the In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/532
Inventor 端青朱虹何君檀华
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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