Polysaccharide pseudo-sponge

A polysaccharide and sponge technology, applied in the field of polysaccharide pseudosponge, can solve problems such as easy cracking of gel sheets, and achieve the effects of high barrier effect, high strength and excellent biodegradability

Inactive Publication Date: 2006-10-18
SEIKAGAKU KOGYO CO LTD
View PDF9 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Also, the problem of brittle gel sheets tends to be exacerbated by their high swelling properties, especially when used as anti-adhesion materials in living tissues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polysaccharide pseudo-sponge
  • Polysaccharide pseudo-sponge
  • Polysaccharide pseudo-sponge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0096] Hereinafter, the present invention is described in more detail through examples, but the examples are only for illustration and are not intended to limit the scope of the present invention. Meanwhile, technical terms and measurement methods used in the Examples are defined as follows.

[0097] (1) Degree of substitution of photoreactive groups

[0098] In the case of introducing a photoreactive group to the carboxyl group of a glycosaminoglycan as a polysaccharide, the degree of substitution of the photoreactive group refers to the number of photoreactive groups introduced per disaccharide repeating unit. percentage. The amount of glycosaminoglycan required to calculate the degree of substitution of photoreactive groups was measured by the carbazole method using its calibration curve. When using cinnamic acid or substituted cinnamic acid as the photoreactive substance, the amount of cinnamic acid was measured by absorbing The measurement method (measurement wavelength...

manufacture Embodiment 1

[0101] Production Example 1 (production of photoreactive hyaluronic acid):

[0102] A mixed solution containing 250 mL of water and 375 mL of dioxane was added to 500 g of 1 wt% sodium hyaluronate (comb-derived product, manufactured by Seikagaku Co., Ltd.; weight-average molecular weight: 900,000) under stirring. in aqueous solution. The resulting mixed solution was mixed successively with the following solutions at room temperature and stirred for 2.5 hours: a solution prepared by dissolving 860 mg of N-hydroxysuccinimide in 2 mL of water (0.6 equivalents / hyaluronic acid disaccharide unit (mol / mol) ), a solution prepared by dissolving 717mg of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDCl / HCl) in 2mL of water (0.3 equivalents / hyaluronic acid disaccharide unit (mol / mol)) and 903mg aminopropyl cinnamate hydrochloride (HCl·H 2 N(CH 2 ) 3 A solution prepared by dissolving OCO-CH=CH-Ph, wherein Ph represents phenyl) in 2 mL of water (0.3 equivalents / disacch...

manufacture Embodiment 2

[0103] Manufacturing example 2 (manufacture of cross-linked hyaluronic acid gel):

[0104] (1) The cinnamic acid-introduced hyaluronic acid obtained in Production Example 1 was dissolved in water for injection to prepare a 4 wt% aqueous solution thereof. The resulting solution was poured into a tempered glass plate mold with a cavity of 6cm x 2.5cm x 1mm (thickness), and then irradiated with ultraviolet light on each surface for 15 minutes under water-cooling conditions using a 3kW metal halide lamp to obtain a transparent Sheet gel (water content: 96wt%). The cross-linking ratio of the obtained gel was determined to be 30%.

[0105] (2) The same procedure as defined in (1) above was carried out, except that cinnamic acid-introduced hyaluronic acid having a degree of substitution of 4.6% was produced using the same method as defined in Production Example 1, thereby obtaining a transparent sheet-like gel . The cross-linking ratio of the resulting gel was determined to be 13....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to view more

Abstract

The present invention provides a photocrosslinked polysaccharide exhibiting low swelling and high in vivo degradability while maintaining appropriate strength. The polysaccharide pseudo-sponge is made by cross-linking reaction of photoreactive polysaccharide obtained by introducing photoreactive groups into the polysaccharide, and exhibits low swelling and low blue dextran dye affinity, which respectively satisfy the following Properties (I) and (II): (I) The swelling ratio does not exceed 125%, which is obtained by immersing a test sample with a thickness of 1mm, a length of 10mm, and a width of 10mm at room temperature and a solvent content of 96wt% in the injection according to the following formula Calculation of the value measured in water for 1 hour: swelling ratio = {(S2-S1)/S1}×100 where S1 represents the area of ​​the test sample before immersion, S2 is the area of ​​the test sample after immersion, and the area is determined by the area of ​​the test sample length and width calculation; and (II) the absorption at 620nm wavelength place is not more than 0.15, it is measured by the aqueous solution containing 0.67wt% polysaccharide, the aqueous solution of this polysaccharide is 1mm thick, 20mm long, 10mm wide, and solvent content is 96 wt% of the test sample was immersed in an aqueous solution containing 0.5 g/mL of blue dextran with a weight average molecular weight of 2,000,000, and then the test sample was washed with water and hydrolyzed to prepare it.

Description

technical field [0001] The present invention relates to polysaccharide pseudosponges, and more particularly to polysaccharide pseudosponges combining sponge and gel properties, which are manufactured by cross-linking reactions of photoreactive polysaccharides obtained by introducing photoreactive groups into polysaccharides . Background technique [0002] Conventionally, there have been known photoreactive polysaccharides obtained by introducing photoreactive groups into polysaccharides, and crosslinked polysaccharides obtained by crosslinking photoreactive polysaccharides by irradiation of light such as ultraviolet rays (for example, Japanese Patent Application Publication (KOKAI) Nos. 6-73102(1994), 8-143604(1996), 9-87236(1997) and 2002-249501). In addition, gels (polysaccharide gels) or sponges (polysaccharide sponges) made of these crosslinked polysaccharides are also known. [0003] Polysaccharide gels have been produced by irradiating photoreactive polysaccharide so...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K9/16A61K47/36A61L31/00C08B11/12C08B37/00C08B37/04
CPCA61K9/1652A61K47/36C08B37/0045C08B37/0084C08B37/00
Inventor 佐藤伦也
Owner SEIKAGAKU KOGYO CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap