Method for controlling quality of ganoderma lucidum spore and ganoderma lucidium spore oil and their preparation

A technique for Ganoderma lucidum spore oil and Ganoderma lucidum spores is applied in the field of quality analysis of medicines or health products, and achieves the effects of strong operability, high reproducibility and simple method.

Active Publication Date: 2006-10-25
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no literature report to use the content of 1,2-oleic acid-3-palmitic acid triglyce...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Research on the method for determining the content of 1,2-oleic acid-3-palmitic triglyceride and triolein in ganoderma lucidum spore oil by high performance liquid chromatography

[0018] (1) Instruments and reagents

[0019] Instrument: Waters2695 high performance liquid chromatography, chromatographic column: KromasilC 18 Column (4.6 mm x 250 mm, 5 μm), Sedex 75 evaporative light scattering detector.

[0020] Reagent: reference substance 1,2-oleic acid-3-palmitic acid triglyceride (abbreviated as OOP, hereinafter the same), glycerol trioleate (abbreviated as OOO, hereinafter the same) are provided by sigma company; Ganoderma lucidum spore oil is provided by Provided by a company in Guangzhou, acetonitrile and isopropanol are chromatographically pure, and other reagents are analytically pure.

[0021] (2) Preparation of need testing solution: precision takes by weighing 62.520mg ganoderma lucidum spore oil, puts in the measuring bottle of 25ml, adds mobile ...

Embodiment 2

[0047] Example 2. Content determination of 1,2-oleic acid-3-palmitic acid triglyceride and triolein in Ganoderma lucidum spore oil emulsion

[0048] Determination by high performance liquid chromatography:

[0049] Chromatographic conditions use octadecyl bonded silica gel as filler; chromatographic column: Kromasil C18, 4.6mm×250mm, 5um column; column temperature: 30°C; mobile phase: acetonitrile: isopropanol with a volume ratio of 40:60 Solution; detected by evaporative light scattering detector; flow rate: 0.5ml / min. The number of theoretical plates is calculated according to the peak of 1,2-oleic acid-3-palmitic acid triglyceride or triglyceride trioleate, both of which are greater than 2000;

[0050] Preparation of reference substance solution: Take 1,2-oleic acid-3-palmitic acid triglyceride and 2.67 mg of triglyceride reference substance, put them in a 25ml measuring bottle, add mobile phase solution to dissolve and dilute to Scale, make solutions containing 0.119mg a...

Embodiment 3

[0053] Example 3 Determination of 1,2-oleic acid-3-palmitic acid triglyceride content in Ganoderma lucidum spore oil by high performance liquid chromatography:

[0054] Chromatographic conditions use octadecyl bonded silica gel as filler; chromatographic column: Kromasil C18, 4.6mm×250mm, 5um column; column temperature: 30°C; mobile phase: acetonitrile: isopropanol with a volume ratio of 60:40 Solution; detected by evaporative light scattering detector; flow rate: 0.8ml / min. The number of theoretical plates is calculated based on the peak of 1,2-oleic acid-3-palmitic acid triglyceride, which is greater than 2000;

[0055] Preparation of reference solution: Take 3.04 mg of 1,2-oleic acid-3-palmitic triglyceride reference substance, put it in a 25ml measuring bottle, add mobile phase solution to dissolve and dilute to the mark, and make each 1ml contain 0.122 mg solution, to obtain the reference substance solution;

[0056] Preparation of the test solution: Accurately weigh 52...

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PUM

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Abstract

The present invention relates to a method for controlling ganoderma spore, ganoderma spore oil and its preparation quality. It is characterized by that it utilizes high performance liquid chromatography to determine 1.2-oleinic acid-3-triglyceride palmitate and/or glycerin trioleate content. Every 1g of ganoderma spore, ganoderma spore oil or its preparation respectively contain 12.0 mg-60.0 mg and 100.0 mg-250.0 mg of 1.2-oleinic acid-3-triglyceride palmitate and/or 8.0 mg-45.0 mg and 80.0 mg-200.0 mg of glycerin trioleate.

Description

technical field [0001] The invention relates to a method for analyzing the quality of medicines or health care products, in particular to a method for determining the content of 1,2-oleic acid-3-palmitic triglyceride or glycerol trioleate in ganoderma lucidum spore oil, ganoderma lucidum spores and preparations thereof . Background technique [0002] Ganoderma lucidum is the general name of Ganoderma lucidium (Leyss.ex Fr.) Karst.] and Zizhi [Ganodermajaponicum (Fr.) Lloyd.] of the genus Ganoderma (Ganoderma) of the family Polyproraceae. Efficacy, known as top grade by "The Classic". Ganoderma lucidium spores are extremely small spores ejected from the cap during the growth and maturity stage of Chizhi. They are the reproductive cells of Ganoderma lucidum and contain all the genetically active substances of Ganoderma lucidum. Their medicinal value is also increasingly valued. The pharmacological effects of Ganoderma lucidum spores mainly include an...

Claims

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Application Information

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IPC IPC(8): A61K36/074G01N30/02G01N33/15
Inventor 李吉来宁德山黄海陈路林
Owner GUANGZHOU HANFANG PHARMA
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