Grifola frondosa strain, culture method and application thereof

A cultivation method, the technology of Grifola frondosa, applied in the field of microorganisms, can solve the problems of difficulty in obtaining strains, unsolved problems of extraction and purification of Grifola frondosa polysaccharides, and high production costs

Active Publication Date: 2006-12-20
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high production cost, the purification problem of extracting Grifola frondosa polysaccharide cannot be solved, especially the strains of Grifola frondosa that are suitable for large-scale fermentation production are always difficult to obtain, and the practical application of Grifola frondosa polysaccharide is progressing slowly

Method used

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  • Grifola frondosa strain, culture method and application thereof
  • Grifola frondosa strain, culture method and application thereof
  • Grifola frondosa strain, culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 bacterial cell culture

[0038]The subculture method adopts slant subculture, and the slant medium adopts potato agar enriched medium, and the ingredients are as follows: 200g potato; 20g glucose; 1g potassium dihydrogen phosphate; 0.5g magnesium sulfate; 20g agar; 1000mL water; 1 Appropriate amount, natural pH.

[0039] The slant culture medium is prepared according to the conventional method; the inoculation culture, according to the requirements of aseptic operation, is inoculated on the ultra-clean bench in the aseptic room, and inoculated by the double-slope inoculation method. After inoculation, culture at 27±1°C for 1 day, you can see creeping hyphae and basal hyphae grow from around the grafted tissue block, and then the creeping hyphae elongate into aerial hyphae, and there are also directly elongate into aerial hyphae. After 4 to 7 days, the young mycelium covered the slope, and the mycelium was white and fluffy, thick and developed, and felt li...

Embodiment 2

[0040] Embodiment 2 Shake flask culture

[0041] Medium for liquid culture: 1% potato, 1% bran, 2% glucose, 0.7% corn flour, 0.3% peptone, 0.14% yeast powder, 0.05% potassium dihydrogen phosphate, and 0.1% magnesium sulfate.

[0042] Prepare the culture medium and put it into the Erlenmeyer flask with a filling coefficient of 0.2-0.4, seal it with a cotton plug, and sterilize it. Grifola frondosa strains are inoculated according to 5-10% (V / V) of the loading amount, and cultured on a shaking table. The temperature is 25-30°C, and the rotation speed is 90-150r / min. After 2 days of culture, a large number of new mycelium balls can be seen, and after 3 to 5 days, a large number of mycelia are formed in the liquid medium, and the mycelium grows in suspension in the liquid medium, and the forms include branched mycelium, flocculated mycelium, and granular fungus Silk.

[0043] Cultivate for 3-5 days according to the above-mentioned method, and the yield of mycelium of Grifola fr...

Embodiment 3

[0044] Embodiment 3 fermentation culture

[0045] For three-stage fermentation, the scale-up ratio of the fermenter is 1:10. The filling factor of the seed tank and fermenter is 50-80%, and the liquid seed inoculation amount is 5-10% (V / V).

[0046] Culture conditions: culture medium C / N is 20-30:1, pH value is 4-7, temperature is 24-29°C, tank pressure is 0.5-1.5Mp, stirring speed is 100-600r / min, dissolved oxygen content is 10-100% .

[0047] The fermenter is gradually enlarged. In the synthetic or semi-synthetic medium containing sufficient nitrogen and carbon sources, the mycelia of Grifola frondosa can grow and reproduce in large quantities under aerobic conditions, so that the amount of mycelia can be continuously accumulated. The growth cycle of the seed tank is 24-48 hours, and the growth cycle of the fermenter is 36-72 hours. The biomass can be increased by hundreds of times within a few days by means of continuous amplification, and a growth cycle can be completed...

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Abstract

The invention discloses a drug and edible two-use fungus grey ramalic GF-932[Grifola frondosa (Dichs.ex Fr.) S.F.Gray] and producing method of grey ramalic beta-dextran, which is characterized by the following: applying in the tablet, soft capsule and hard capsule, liquid drugs injection and freeze dried; reserving in the China biological bacterial reserving management committee common microbe center with CGMCC No.1709 as reserving number.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to a kind of biological material——Grifola frondosa strain, a microorganism whose fermentation product and its extract have medicinal functions, and its polysaccharides in Grifola frondosa, especially Grifola frondosa Application in the production of β-glucan, capsules such as tablets, soft capsules and hard capsules, injections such as water injections and freeze-dried powder injections. Background technique [0002] Since the Japanese scientist Chihara Goro discovered that lentinan has anti-tumor activity in 1969, scientists from various countries have devoted themselves to the research of fungal polysaccharides in the field of anti-tumor, created a new field of tumor biological therapy, especially immunotherapy, and made outstanding progress. Among the fungal polysaccharides, a large number of polysaccharide anti-tumor preparations with significant curative effects ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/08A61P35/00A61P31/00A61K31/716A61K9/20A61K9/48
Inventor 徐泽平王宝琴
Owner CHAMBROAD CHEM IND RES INST CO LTD
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